Cellulomonas fulva sp. nov., isolated from oil- contaminated soil

초록

A yellow- coloured, Gram- stain- positive, motile, aerobic and rod- shaped bacteria, designated DKR- 3T, was isolated from oil- contaminated experimental soil. Strain DKR- 3T could grow at pH 5.0-10.5 (optimum, pH 7.0-8.5), at 10-40 degrees C (optimum, 25-32 degrees C) and tolerated 3.5 % of NaCl. Phylogenetic analyses based on its 16S rRNA gene sequence indicated that strain DKR3T formed a lineage within the family Cellulomonadaceae and was clustered with members of the genus Cellulomonas. Strain DKR-3T had highest 16S rRNA gene sequence similarities to Cellulomonas gelida DSM 20111T (98.3 %), Cellulomonas persica JCM 18111T (98.2 %) and Cellulomonas uda DSM 20107T (97.8 %). The predominant respiratory quinone was tetrahydrogenated menaquinone with nine isoprene units [MK- 9(H4)]. The principal cellular fatty acids were anteiso- C15:0, C16: 0 and anteiso- C17:0. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The cell- wall diamino acid was iota-ornithine whereas rhamnose and glucose were the cell- wall sugars. The DNA G+C content was 74.2mol %. The genome of strain DKR- 3T was 3.74 Mb and contained three putative biosynthetic gene clusters. The average nucleotide identity and digital DNA-DNA hybridization relatedness values between strain DKR- 3T and its phylogenetically related members were below the species threshold values. Based on a polyphasic study, strain DKR- 3T represents a novel species belonging to the genus Cellulomonas, for which the name Cellulomonas fulva sp. nov. is proposed. The type strain is DKR- 3T (=KACC 22071T=NBRC 114730T).

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