Selective enrichment of cysteine-containing peptides using SPDP-Functionalized superparamagnetic Fe3O4@SiO2 nanoparticles: Application to comprehensive proteomic profiling

Palani, Arudra; Lee, Je-Sun; Huh, Jiyoung; Kim, Minsik; Lee, Yeon-Ji; Chang, Jeong Ho; Lee, Kwangyeol; Lee, Sang-Won

doi:10.1021/pr800067x

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Selective enrichment of cysteine-containing peptides using SPDP-Functionalized superparamagnetic Fe3O4@SiO2 nanoparticles: Application to comprehensive proteomic profiling

Palani, Arudra;
Lee, Je-Sun;
Huh, Jiyoung;
Kim, Minsik;
Lee, Yeon-Ji;
... Lee, Kwangyeol;
... Lee, Sang-Won;
외 1명

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42

초록

Superparamagnetic Fe3O4@SiO2 core-shell nanoparticles (ca. 30 nm diameter), which are surface-modified with a thiol-specific functional group, exhibit superb capturing efficiency toward cysteinyl peptides without contamination from nonspecifically interacting peptides, as clearly evidenced through LC/MS/MS analysis.

키워드

superparamagnetic nanoparticle; surface modification; cysteine containing peptide enrichment; tandem mass spectrometry; proteomics; MASS-SPECTROMETRY; NANOCRYSTALS

제목: Selective enrichment of cysteine-containing peptides using SPDP-Functionalized superparamagnetic Fe3O4@SiO2 nanoparticles: Application to comprehensive proteomic profiling

저자: Palani, Arudra; Lee, Je-Sun; Huh, Jiyoung; Kim, Minsik; Lee, Yeon-Ji; Chang, Jeong Ho; Lee, Kwangyeol; Lee, Sang-Won

DOI: 10.1021/pr800067x

발행일: 2008-08

유형: Article

저널명: Journal of Proteome Research

권: 7

호: 8

페이지: 3591 ~ 3596