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초록
Structured phosphatidylcholine (SPC) was successfully produced via immobilized phospholipase A(1) (PLA(1)) - catalyzed acidolysis of phosphatidylcholine (PC) with conjugated linoleic acid (CLA). The effects of enzyme loading (2, 5, 10, 15 and 20%, with respect to the weight of substrates), temperature (20, 30, 40, 50 and 60 degrees C) and the molar ratio of substrates (1:2, 1:4, 1:6, 1:8 and 1:10, PC/CLA) were evaluated to maximize the incorporation of CLA into PC. The maximum incorporation of CLA achieved was ca. 90% for 24 h of reaction at 50 degrees C and 200 rpm, using a 1:4 substrate molar ratio and an enzyme loading of 15%.
키워드
Acidolysis; Conjugated linoleic acid; Phosphatidylcholine; Phospholipase A(1); Structured phospholipids; LIPASE-CATALYZED ACIDOLYSIS; POLYUNSATURATED FATTY-ACIDS; LECITHIN; TRANSESTERIFICATION; ESTERIFICATION; OPTIMIZATION; ENRICHMENT
- 제목
- Use of immobilized phospholipase A(1)catalyzed acidolysis for the production of structured phosphatidylcholine with an elevated conjugated linoleic acid content
- 저자
- Baeza-Jimenez, R.; Gonzalez-Rodriguez, J.; Kim, In-H.; Garcia, H. S.; Otero, C.
- 발행일
- 2012-01
- 유형
- Article
- 저널명
- Grasas y Aceites
- 권
- 63
- 호
- 1
- 페이지
- 44 ~ 52