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A highly sensitive immunoassay using antibody-conjugated spherical mesoporous silica with immobilized enzymes
- Eum, Ji Young;
- Hwang, Sang Youn;
- Ju, Youngjun;
- Shim, Jong Min;
- Piao, Yunxian;
- ... Kim, Jungbae;
- 외 2명
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19초록
A highly sensitive immunoassay was developed by using antibody-conjugated spherical mesoporous silica with immobilized enzymes. The higher ratio of enzyme/antibody than conventional ELISA improved both the sensitivity and dynamic range. Especially, the use of spherical mesoporous silica could achieve a limit of detection (LOD) with a sensitivity that is 20 times more than that of ELISA using amorphous silica.
키워드
ELECTROCHEMICAL IMMUNOASSAY; BIOFUEL CELLS; NANOBIOCATALYSIS; ASSAY; DNA
- 제목
- A highly sensitive immunoassay using antibody-conjugated spherical mesoporous silica with immobilized enzymes
- 저자
- Eum, Ji Young; Hwang, Sang Youn; Ju, Youngjun; Shim, Jong Min; Piao, Yunxian; Lee, Jinwoo; Kim, Hak-Sung; Kim, Jungbae
- 발행일
- 2014
- 유형
- Article
- 권
- 50
- 호
- 27
- 페이지
- 3546 ~ 3548
- 언어
- ENG
- 출판사
- ROYAL SOC CHEMISTRY
- 발행국가
- 영국
- 분량
- 3 페이지
- ISSN
- E 1364-548X
P 1359-7345