Plasma-based diagnostic and screening platform using a combination of biosensing signals in Alzheimer?s disease

초록

Using biosensor to screen for Alzheimer's disease (AD) facilitates early detection of AD with high sensitivity and accuracy. This approach overcomes the limitations of conventional AD diagnostic methods, such as neuropsy-chological assessment and neuroimaging analysis. Here, we propose a simultaneous analysis of signal combi-nations generated by four crucial AD biomarkers (Amyloid beta 1-40 (A beta 40), A beta 42, total tau 441 (tTau441), and phosphorylated tau 181 (pTau181)) by inducing a dielectrophoretic (DEP) force on fabricated interdigitated microelectrode (IME) sensor. By applying an optimal DEP force, our biosensor selectively concentrates and filters the plasma-based AD biomarkers, exhibiting high sensitivity (limit of detection <100 fM) and selectivity in plasma-based AD biomarkers detection (p < 0.0001). Consequently, it is demonstrated that a complex combined signal comprising four AD-specific biomarker signals (A beta 40 -A beta 42 + tTau441 -pTau181) can differentiate tween patients with AD and healthy subjects with high accuracy (78.85%) and precision (80.95%) (p < 0.0001).

키워드