Sphingomicrobium sediminis sp. nov., isolated from marine sediment in the Republic of Korea

초록

A Gram- stain- negative, rod- shaped, bright- orange coloured bacterium without flagellum, designated as strain GRR- S6- 50(T), was isolated from a tidal flat of Garorim bay, Taean- gun, Chungcheongbuk- do, Republic of Korea. Cells grew aerobically at 20-37 degrees C (optimum, 30 degrees C), pH 7.0-10.0 (optimum, pH 7.0) and with 1-5 % (w/v) NaCl (optimum, 3 %). The 16S rRNA gene sequence analysis demonstrated that strain GRR- S6- 50(T) was closely related to Sphingomicrobium aestuariivivum AH-M8T with a sequence similarity of 97.80 % followed by Sphingomicrobium astaxanthinifaciens CC-AMO-30BT (97.44 %), Sphingomicrobium marinum CCAMZ-30MT (97.16 %), Sphingomicrobium arenosum CAU 1457(T) (96.37 %), Sphingomicrobium flavum CC-AMZ-30NT (95.31 %) and Sphingomicrobium lutaoense CC-TBT-3(T) (95.23 %). The average nucleotide identity and digital DNA-DNA hybridization values with related strains ranged from 74.5 to 77.3% and 21.1 to 35.0 %, respectively. The G+C content of strain GRR- S6- 50T was 63.30 mol%. The strain has ubiquinone- 10 as the predominant respiratory quinone and the major fatty acids were C18:3 omega 6c (54.57 %) and C17: 1 omega 6c (10.58 %). The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, three unidentified lipids and one glycolipid. On the basis of the results of phylogenetic, phenotypic and chemotaxonomic studies, strain GRRS6-50(T) is regarded to represent a novel species within the genus Sphingomicrobium, for which the name Sphingomicrobium sediminis sp. nov. (KACC 22562(T)=KCTC 92123(T)=JCM 35084(T)) is proposed.

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