Recent Advances in Loop-Mediated Isothermal Amplification (LAMP) for On-Site Virus Detection

초록

The COVID-19 pandemic underscored the urgent need for rapid identification and isolation of infected individuals, thereby accelerating the demand for on-site virus detection technologies. Although polymerase chain reaction (PCR) remains the “gold standard” for viral diagnostics, its dependence on bulky instrumentation, trained personnel, and lengthy processing times limits its applicability in on-site and point-of-care testing (POCT) settings, where speed and simplicity are paramount. To overcome these challenges, loop-mediated isothermal amplification (LAMP) has emerged as a promising alternative, enabling rapid nucleic acid amplification under isothermal conditions. However, conventional tube-based LAMP still involves manual operations such as reagent mixing and sample handling, which restrict its usability outside of laboratory environments. Consequently, the development of integrated LAMP platforms capable of performing sample preparation, amplification, and detection in a streamlined and automated manner, delivering rapid results, has become a critical goal for on-site virus detection. In this review, we highlight recent advances in onsite LAMP-based virus detection platforms employing paper, microfluidics, and hydrogels. Furthermore, we discuss current technical limitations, perspectives, and future directions, including automation, multiplexing, and machine learning (ML)-assisted readouts. © 2025 Elsevier B.V., All rights reserved.

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