Post-translational regulation of SHORT VEGETATIVE PHASE as a major mechanism for thermoregulation of flowering
- Authors
- Lee, J.H.; Chung, K.S.; Kim, S.-K.; Ahn, J.H.
- Issue Date
- 2014
- Publisher
- Landes Bioscience
- Keywords
- Alternative splicing; Ambient temperature; Ambient temperature-responsive flowering; FLM-β; FLM-δ; SVP; Thermoregulation
- Citation
- Plant Signaling and Behavior, v.9, no.MAR
- Indexed
- SCOPUS
- Journal Title
- Plant Signaling and Behavior
- Volume
- 9
- Number
- MAR
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/100712
- DOI
- 10.4161/psb.28193
- ISSN
- 1559-2316
- Abstract
- In contrast to our extensive knowledge of vernalization, we know relatively little about the regulation of ambient temperature-responsive flowering. Recent reports revealed that FLOWERING LOCUS M (FLM) and SHORT VEGETATIVE PHASE (SVP) regulate high ambient temperature-responsive flowering through two different mechanisms: degradation of SVP protein and formation of a non-functional SVP-FLM-δ complex. To investigate further the mechanism of thermoregulation of flowering, we performed real-time quantitative polymerase chain reaction (RT -qPCR) and in vitro pull-down assays. We found that FLM-β and FLM-δ transcripts show similar absolute levels at different temperatures. Also, His-SVP protein bound to the GST-FLM-β or -δ proteins with similar binding intensities. These results suggest that functional SVP-FLM-β and non-functional SVP-FLM-δ complexes form similarly at warmer temperatures, thus indicating that posttranslational regulation of SVP functions as a major mechanism for thermoregulation in flowering. © 2014 Landes Bioscience.
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