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Inhibitory effects of the kappa-casein macropeptide isolated from milk protein on the biofilm formation and virulence of Listeria monocytogenes

Authors
Yun, Hyun SunKim, YounghoonPark, Mi RiKim, Sae HunOh, Sejong
Issue Date
2014
Publisher
TAYLOR & FRANCIS LTD
Keywords
biofilm formation; Caenorhabditis elegans; kappa-casein macropeptide; Listeria monocytogenes; two-dimensional gel electrophoresis
Citation
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, v.78, no.3, pp.490 - 498
Indexed
SCIE
SCOPUS
Journal Title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume
78
Number
3
Start Page
490
End Page
498
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/101032
DOI
10.1080/09168451.2014.885829
ISSN
0916-8451
Abstract
We demonstrate the inhibitory effects of kappa-casein macropeptide (CMP) on the biofilm formation and virulence of Listeria monocytogenes Scott A. The inhibition of biofilm formation by CMP was initially investigated by using the protocol applied for the 96-well microtiter plate assay. Low concentrations of CMP (0.1, 0.2, 0.3, 0.4, and 0.5 mg/mL) that were tested resulted in a profound inhibitory effect on biofilm formation at a concentration of 0.4 mg/mL. CMP also significantly repressed the transcription of inlA (encoding internalin A) that was responsible for the initial adhesion and invasion event, and prolonged the survival of Caenorhabditis elegans infected by L. monocytogenes. Two-dimensional gel electrophoresis showed that newly identified proteins in the presence of CMP were involved in the stress response and metabolic processes that have important roles in developing listerial biofilms. Our results suggest that CMP from milk protein would be capable of eliminating biofilm formation and virulence by L. monocytogenes in the food industry.
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