Uniform tricalcium phosphate beads with an open porous structure for tissue engineering
- Authors
- Ryu, Tae-Kyung; Oh, Myeong-Jin; Moon, Seung-Kwan; Paik, Dong-Hyun; Kim, Sung-Eun; Park, Jong-Hoon; Choi, Sung-Wook
- Issue Date
- 1-12월-2013
- Publisher
- ELSEVIER
- Keywords
- Tricalcium phosphate; Porous structure; Tissue penetration; Tissue engineering
- Citation
- COLLOIDS AND SURFACES B-BIOINTERFACES, v.112, pp.368 - 373
- Indexed
- SCIE
SCOPUS
- Journal Title
- COLLOIDS AND SURFACES B-BIOINTERFACES
- Volume
- 112
- Start Page
- 368
- End Page
- 373
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/101333
- DOI
- 10.1016/j.colsurfb.2013.08.023
- ISSN
- 0927-7765
- Abstract
- Uniform tricalcium phosphate (TCP) porous beads with micro and macro pore sizes were fabricated using a simple fluidic device. For micro-porous TCP beads, an aqueous gelatin mixture containing TCP powder was introduced as the discontinuous phase into the fluidic device, where a toluene phase served as the continuous phase. The resulting aqueous TCP droplets were instantly frozen at -20 degrees C and freezedried, followed by calcination at 1200 degrees C. An oil-in-water-in-oil (O/W/O) emulsion templating method was employed to fabricate macro-porous TCP beads. An oil-in-water (O/W) emulsion was introduced into the fluidic device as the discontinuous phase with all other experimental conditions the same as for the micro-porous TCP beads. Uniform macro-porous TCP beads with a highly porous structure were finally obtained after freeze-drying and calcination. Large pore size and good interconnectivity of the macro-porous TCP beads were confirmed by scanning electron microscopy and porosimetry. In addition, penetration of host tissue into the macro-pores of the TCP beads was demonstrated by subcutaneously implanting the two types of porous TCP beads into mice and histologically analyzing stained sections at 1-4 weeks post implantation. The macro-porous TCP beads with a highly open porous structure could potentially be used as an injectable material for bone tissue engineering. (C) 2013 Elsevier B.V. All rights reserved.
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Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
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