UVB Induces HIF-1 alpha-Dependent TSLP Expression via the JNK and ERK Pathways
- Authors
- Jang, Yeonsue; Jeong, Sang H.; Park, Yoon-Hee; Bae, Hyun C.; Lee, Hana; Ryu, Woo-In; Park, Gil H.; Son, Sang W.
- Issue Date
- 11월-2013
- Publisher
- ELSEVIER SCIENCE INC
- Citation
- JOURNAL OF INVESTIGATIVE DERMATOLOGY, v.133, no.11, pp.2601 - 2608
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF INVESTIGATIVE DERMATOLOGY
- Volume
- 133
- Number
- 11
- Start Page
- 2601
- End Page
- 2608
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/101671
- DOI
- 10.1038/jid.2013.203
- ISSN
- 0022-202X
- Abstract
- Thymic stromal lymphopoietin (TSLP) may have a key role in the initiation and maintenance of allergic inflammatory diseases, including atopic dermatitis. The present study revealed that UVB radiation exposure could induce TSLP expression in human keratinocytes and a human skin equivalent model. In addition, we investigated the regulatory mechanism of UVB-induced TSLP expression in keratinocytes. TSLP expression was upregulated by transfection with pcDNA3-hypoxia-inducible factor (HIF)-1 alpha (P402A and P564A), which stably expresses HIF-1 alpha protein. UVB-induced TSLP induction in keratinocytes was suppressed in the treatment of mitogen-activated protein kinase inhibitors or small interfering RNAs against HIF-1 alpha. The results of chromatin immunoprecipitation assays indicate the direct involvement of HIF-1 alpha in UVB-mediated TSLP induction. Taken together, these findings indicate that UVB exposure may increase TSLP expression through a HIF-1 alpha-dependent mechanism via the c-JUN N-terminal kinase and extracellular signal-regulated kinase pathways in human keratinocytes. Our data showed that UVB-induced TSLP might increase secretion of the T-helper type 2-attracting chemokine (c-c motif) ligand 17 by human dendritic cells. The present study suggests an important role of HIF-1 alpha in UVB-mediated immune response in keratinocytes.
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Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
- Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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