Rck1 up-regulates Hog1 activity by down-regulating Slt2 activity in Saccharomyces cerevisiae
DC Field | Value | Language |
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dc.contributor.author | Chang, Miwha | - |
dc.contributor.author | Kang, Hyun-Jun | - |
dc.contributor.author | Baek, In-Joon | - |
dc.contributor.author | Kang, Chang-Min | - |
dc.contributor.author | Park, Yong-Sung | - |
dc.contributor.author | Yun, Cheol-Won | - |
dc.date.accessioned | 2021-09-05T20:14:20Z | - |
dc.date.available | 2021-09-05T20:14:20Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2013-10-11 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/101879 | - |
dc.description.abstract | We previously reported that the over-expression of KDX1 up-regulates RCK1 gene expression. To further understand the function of Rck1, microarray analysis was performed using a RCK1 over-expressing strain. Based on microarray and Northern blot analyses, we determined that the expression of KDX1 was downregulated when RCK1 was over-expressed. Furthermore, we determined that phosphorylated forms of Slt2 and Mkk2 were down-regulated by the over-expression of RCK1. Ptp2, a phosphatase that is regulated by the Slt2 MAP kinase pathway, was down-regulated by the over-expression of RCK1. Ptp2 is a negative regulator of Hog1; thus, the phosphorylated form of Hog1 was up-regulated by RCK1 overexpression. A point mutation of lysine 152 to arginine resulted in a failure to up-regulate Hog1 and the subsequent down-regulation of CTT1, which is a Hog1 pathway target gene. Furthermore, using microarray and Northern blot analyses, we determined that genes that are regulated by Msn2/Msn4 were up-regulated by Rck1 and that this was the result of Hog1 activation by RCK1 over-expression. Together, our results suggest that Rck1 inhibits Slt2 MAP kinase pathway activity and then Ptp2, which subsequently activates Hog1. (C) 2013 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | ACTIVATED PROTEIN-KINASE | - |
dc.subject | MAP KINASE | - |
dc.subject | CELL INTEGRITY | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | TRANSCRIPTIONAL RESPONSE | - |
dc.subject | TYROSINE PHOSPHATASES | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | PATHWAY | - |
dc.subject | YEAST | - |
dc.subject | OSMOSTRESS | - |
dc.title | Rck1 up-regulates Hog1 activity by down-regulating Slt2 activity in Saccharomyces cerevisiae | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yun, Cheol-Won | - |
dc.identifier.doi | 10.1016/j.bbrc.2013.09.045 | - |
dc.identifier.scopusid | 2-s2.0-84885384732 | - |
dc.identifier.wosid | 000326064900020 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.440, no.1, pp.119 - 124 | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 440 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 119 | - |
dc.citation.endPage | 124 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.subject.keywordPlus | ACTIVATED PROTEIN-KINASE | - |
dc.subject.keywordPlus | MAP KINASE | - |
dc.subject.keywordPlus | CELL INTEGRITY | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | TRANSCRIPTIONAL RESPONSE | - |
dc.subject.keywordPlus | TYROSINE PHOSPHATASES | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | YEAST | - |
dc.subject.keywordPlus | OSMOSTRESS | - |
dc.subject.keywordAuthor | Saccharomyces cerevisiae | - |
dc.subject.keywordAuthor | Kdx1 | - |
dc.subject.keywordAuthor | Rck1 | - |
dc.subject.keywordAuthor | Slt2 | - |
dc.subject.keywordAuthor | Mkk2 | - |
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