Rck1 up-regulates Hog1 activity by down-regulating Slt2 activity in Saccharomyces cerevisiae
- Authors
- Chang, Miwha; Kang, Hyun-Jun; Baek, In-Joon; Kang, Chang-Min; Park, Yong-Sung; Yun, Cheol-Won
- Issue Date
- 11-10월-2013
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- Saccharomyces cerevisiae; Kdx1; Rck1; Slt2; Mkk2
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.440, no.1, pp.119 - 124
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- Volume
- 440
- Number
- 1
- Start Page
- 119
- End Page
- 124
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/101879
- DOI
- 10.1016/j.bbrc.2013.09.045
- ISSN
- 0006-291X
- Abstract
- We previously reported that the over-expression of KDX1 up-regulates RCK1 gene expression. To further understand the function of Rck1, microarray analysis was performed using a RCK1 over-expressing strain. Based on microarray and Northern blot analyses, we determined that the expression of KDX1 was downregulated when RCK1 was over-expressed. Furthermore, we determined that phosphorylated forms of Slt2 and Mkk2 were down-regulated by the over-expression of RCK1. Ptp2, a phosphatase that is regulated by the Slt2 MAP kinase pathway, was down-regulated by the over-expression of RCK1. Ptp2 is a negative regulator of Hog1; thus, the phosphorylated form of Hog1 was up-regulated by RCK1 overexpression. A point mutation of lysine 152 to arginine resulted in a failure to up-regulate Hog1 and the subsequent down-regulation of CTT1, which is a Hog1 pathway target gene. Furthermore, using microarray and Northern blot analyses, we determined that genes that are regulated by Msn2/Msn4 were up-regulated by Rck1 and that this was the result of Hog1 activation by RCK1 over-expression. Together, our results suggest that Rck1 inhibits Slt2 MAP kinase pathway activity and then Ptp2, which subsequently activates Hog1. (C) 2013 Elsevier Inc. All rights reserved.
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