Effect of hyaluronic acid (HA) in a HA/PLGA scaffold on annulus fibrosus regeneration: In vivo tests
- Authors
- Song, Jeong Eun; Kim, Min Jeong; Yoon, Hyeon; Yoo, Hanna; Lee, Yu Jeong; Kim, Ha Neul; Lee, Dongwon; Yuk, Soon Hong; Khang, Gilson
- Issue Date
- 10월-2013
- Publisher
- POLYMER SOC KOREA
- Keywords
- poly(lactide-co-glycolide) (PLGA); hyaluronic acid (HA); annulus fibrosus; in vivo
- Citation
- MACROMOLECULAR RESEARCH, v.21, no.10, pp.1075 - 1082
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- MACROMOLECULAR RESEARCH
- Volume
- 21
- Number
- 10
- Start Page
- 1075
- End Page
- 1082
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/101981
- DOI
- 10.1007/s13233-013-1137-z
- ISSN
- 1598-5032
- Abstract
- Intervertebral disc (IVD) degeneration is an age-related process that affects the biomechanical properties of the spine and is assumed to be one of the principal causes of low back pain. Poly(lactide-co-glycolide) (PLGA) and hyaluronic acid (HA) have been widely used as biocompatible scaffold materials for tissue regeneration. In the present study, we fabricated a microporous PLGA scaffold by the salt-leaching method and HA-loaded PLGA scaffolds by the penetrating method. As the porosity of PLGA and PLGA/HA is 90.7% and 96.5%, respectively, PLGA/HA scaffold has the higher porosity. However, the pore size of PLGA (240.7 +/- 14.0 mu m) is the larger than HA/PLGA (212.0 +/- 12.5 mu m), and cells from the annulus fibrosus (AF) were seeded into the scaffolds. For the in vivo study, the PLGA and HA/PLGA scaffolds were implanted in four-week-old nude mice. All scaffolds were characterized using a scanning electron microscope (SEM), and the amounts of glycosaminoglycan (GAG) and collagen in the scaffolds were determined by a spectrophotometer. Histological evaluation indicated the higher level of AF cell proliferation in the HA/PLGA scaffold than in PLGA scaffolds alone. In addition, AF cells showed the stronger production of GAG and collagen in HA/PLGA. Our results indicate that the HA/PLGA scaffold might be useful for intervertebral disc regeneration.
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Collections - College of Pharmacy > Department of Pharmaceutical Science > 1. Journal Articles
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