Effects of Ginkgo biloba Extract on Cultured Human Retinal Pigment Epithelial Cells under Chemical Hypoxia
DC Field | Value | Language |
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dc.contributor.author | Oh, Jong-Hyun | - |
dc.contributor.author | Oh, Jaeryung | - |
dc.contributor.author | Togloom, Ariunaa | - |
dc.contributor.author | Kim, Seong-Woo | - |
dc.contributor.author | Huh, Kuhl | - |
dc.date.accessioned | 2021-09-05T20:38:26Z | - |
dc.date.available | 2021-09-05T20:38:26Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2013-10 | - |
dc.identifier.issn | 0271-3683 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/101999 | - |
dc.description.abstract | Purpose: To investigate the effects of Ginkgo biloba extract (GBE) on expression of hypoxia-inducible factor (HIF)-1 alpha and vascular endothelial growth factor (VEGF) in retinal pigment epithelial (RPE) cells under chemical hypoxia. Materials and Methods: RPE cells (ARPE-19) were cultured in either untreated media (control group), media treated with 200 mu M cobalt chloride (hypoxia group) or media treated with both 200 mu M cobalt chloride and 100 mu g/ml GBE (hypoxia+GBE group) for various amounts of time. HIF-1 alpha and VEGF expression were compared between groups. HIF-1 alpha and VEGF messenger RNA (mRNA) were quantified using real-time polymerase chain reaction (PCR). HIF-1 alpha of extracted nuclei and VEGF of the media were quantified using enzyme-linked immunosorbent assay (ELISA). The expression of HIF-1 alpha was also assessed with Western blot and immunocytochemistry. Results: HIF-1 alpha mRNA, VEGF mRNA, HIF-1 alpha and VEGF levels were higher in the hypoxia group compared with the control group; however, real-time PCR revealed decreased expression of HIF-1 alpha mRNA and VEGF mRNA in the hypoxia+GBE group compared with the hypoxia group. In the ELISA, the HIF-1 alpha and VEGF protein concentrations also decreased with GBE treatment. Western blot and immunostaining showed that the intensity of HIF-1 alpha signals in the hypoxia+GBE group was lower than that of the hypoxia group. Conclusion: GBE reduced HIF-1 alpha and VEGF expression in RPE cells cultured under chemical hypoxia in vitro. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | INFORMA HEALTHCARE | - |
dc.subject | ENDOTHELIAL GROWTH-FACTOR | - |
dc.subject | HUMAN RPE CELLS | - |
dc.subject | PLATELET-ACTIVATING-FACTOR | - |
dc.subject | MACULAR DEGENERATION AMD | - |
dc.subject | AGE-RELATED MACULOPATHY | - |
dc.subject | TARGETING HIF-1-ALPHA | - |
dc.subject | INDUCIBLE FACTORS | - |
dc.subject | FACTOR EXPRESSION | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | EYE DISEASE | - |
dc.title | Effects of Ginkgo biloba Extract on Cultured Human Retinal Pigment Epithelial Cells under Chemical Hypoxia | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Jaeryung | - |
dc.contributor.affiliatedAuthor | Kim, Seong-Woo | - |
dc.contributor.affiliatedAuthor | Huh, Kuhl | - |
dc.identifier.doi | 10.3109/02713683.2013.804093 | - |
dc.identifier.scopusid | 2-s2.0-84883191569 | - |
dc.identifier.wosid | 000323719500009 | - |
dc.identifier.bibliographicCitation | CURRENT EYE RESEARCH, v.38, no.10, pp.1072 - 1082 | - |
dc.relation.isPartOf | CURRENT EYE RESEARCH | - |
dc.citation.title | CURRENT EYE RESEARCH | - |
dc.citation.volume | 38 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 1072 | - |
dc.citation.endPage | 1082 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Ophthalmology | - |
dc.relation.journalWebOfScienceCategory | Ophthalmology | - |
dc.subject.keywordPlus | ENDOTHELIAL GROWTH-FACTOR | - |
dc.subject.keywordPlus | HUMAN RPE CELLS | - |
dc.subject.keywordPlus | PLATELET-ACTIVATING-FACTOR | - |
dc.subject.keywordPlus | MACULAR DEGENERATION AMD | - |
dc.subject.keywordPlus | AGE-RELATED MACULOPATHY | - |
dc.subject.keywordPlus | TARGETING HIF-1-ALPHA | - |
dc.subject.keywordPlus | INDUCIBLE FACTORS | - |
dc.subject.keywordPlus | FACTOR EXPRESSION | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | EYE DISEASE | - |
dc.subject.keywordAuthor | Chemical hypoxia | - |
dc.subject.keywordAuthor | hypoxia-inducible factor-1 alpha | - |
dc.subject.keywordAuthor | Ginkgo biloba extract | - |
dc.subject.keywordAuthor | retinal pigment epithelial cell | - |
dc.subject.keywordAuthor | vascular endothelial growth factor | - |
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