Effects of Ginkgo biloba Extract on Cultured Human Retinal Pigment Epithelial Cells under Chemical Hypoxia
- Authors
- Oh, Jong-Hyun; Oh, Jaeryung; Togloom, Ariunaa; Kim, Seong-Woo; Huh, Kuhl
- Issue Date
- 10월-2013
- Publisher
- INFORMA HEALTHCARE
- Keywords
- Chemical hypoxia; hypoxia-inducible factor-1 alpha; Ginkgo biloba extract; retinal pigment epithelial cell; vascular endothelial growth factor
- Citation
- CURRENT EYE RESEARCH, v.38, no.10, pp.1072 - 1082
- Indexed
- SCIE
SCOPUS
- Journal Title
- CURRENT EYE RESEARCH
- Volume
- 38
- Number
- 10
- Start Page
- 1072
- End Page
- 1082
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/101999
- DOI
- 10.3109/02713683.2013.804093
- ISSN
- 0271-3683
- Abstract
- Purpose: To investigate the effects of Ginkgo biloba extract (GBE) on expression of hypoxia-inducible factor (HIF)-1 alpha and vascular endothelial growth factor (VEGF) in retinal pigment epithelial (RPE) cells under chemical hypoxia. Materials and Methods: RPE cells (ARPE-19) were cultured in either untreated media (control group), media treated with 200 mu M cobalt chloride (hypoxia group) or media treated with both 200 mu M cobalt chloride and 100 mu g/ml GBE (hypoxia+GBE group) for various amounts of time. HIF-1 alpha and VEGF expression were compared between groups. HIF-1 alpha and VEGF messenger RNA (mRNA) were quantified using real-time polymerase chain reaction (PCR). HIF-1 alpha of extracted nuclei and VEGF of the media were quantified using enzyme-linked immunosorbent assay (ELISA). The expression of HIF-1 alpha was also assessed with Western blot and immunocytochemistry. Results: HIF-1 alpha mRNA, VEGF mRNA, HIF-1 alpha and VEGF levels were higher in the hypoxia group compared with the control group; however, real-time PCR revealed decreased expression of HIF-1 alpha mRNA and VEGF mRNA in the hypoxia+GBE group compared with the hypoxia group. In the ELISA, the HIF-1 alpha and VEGF protein concentrations also decreased with GBE treatment. Western blot and immunostaining showed that the intensity of HIF-1 alpha signals in the hypoxia+GBE group was lower than that of the hypoxia group. Conclusion: GBE reduced HIF-1 alpha and VEGF expression in RPE cells cultured under chemical hypoxia in vitro.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.