A microfluidic array for quantitative analysis of human neural stem cell self-renewal and differentiation in three-dimensional hypoxic microenvironment
DC Field | Value | Language |
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dc.contributor.author | Yang, Kisuk | - |
dc.contributor.author | Han, Sewoon | - |
dc.contributor.author | Shin, Yoojin | - |
dc.contributor.author | Ko, Eunkyung | - |
dc.contributor.author | Kim, Jin | - |
dc.contributor.author | Park, Kook In | - |
dc.contributor.author | Chung, Seok | - |
dc.contributor.author | Cho, Seung-Woo | - |
dc.date.accessioned | 2021-09-05T22:00:14Z | - |
dc.date.available | 2021-09-05T22:00:14Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2013-09 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/102229 | - |
dc.description.abstract | We report a microfluidic array for investigating and quantitatively analyzing human neural stem cell (hNSC) self-renewal and differentiation in an in vivo-like microenvironment. NSC niche conditions, including three-dimensional (3D) extracellular matrices and low oxygen tension, were effectively reconstituted in the microfluidic array in a combinatorial manner. The array device was fabricated to be detachable, rendering it compatible with quantitative real-time polymerase chain reaction for quantifying the effects of the biomimetic conditions on hNSC self-renewal and differentiation. We show that throughput of 3D cell culture and quantitative analysis can be increased. We also show that 3D hypoxic microenvironments maintain hNSC self-renewal capacity and direct neuronal commitment during hNSC differentiation. (C) 2013 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | HUMAN NEURODEGENERATIVE DISORDERS | - |
dc.subject | EXTRACELLULAR-MATRIX | - |
dc.subject | DOPAMINERGIC DIFFERENTIATION | - |
dc.subject | VASCULAR NICHE | - |
dc.subject | PROLIFERATION | - |
dc.subject | OXYGEN | - |
dc.subject | CULTURE | - |
dc.subject | HYDROXYLATION | - |
dc.subject | NEUROGENESIS | - |
dc.subject | PLURIPOTENCY | - |
dc.title | A microfluidic array for quantitative analysis of human neural stem cell self-renewal and differentiation in three-dimensional hypoxic microenvironment | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chung, Seok | - |
dc.identifier.doi | 10.1016/j.biomaterials.2013.05.067 | - |
dc.identifier.scopusid | 2-s2.0-84879461661 | - |
dc.identifier.wosid | 000322049200007 | - |
dc.identifier.bibliographicCitation | BIOMATERIALS, v.34, no.28, pp.6607 - 6614 | - |
dc.relation.isPartOf | BIOMATERIALS | - |
dc.citation.title | BIOMATERIALS | - |
dc.citation.volume | 34 | - |
dc.citation.number | 28 | - |
dc.citation.startPage | 6607 | - |
dc.citation.endPage | 6614 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | HUMAN NEURODEGENERATIVE DISORDERS | - |
dc.subject.keywordPlus | EXTRACELLULAR-MATRIX | - |
dc.subject.keywordPlus | DOPAMINERGIC DIFFERENTIATION | - |
dc.subject.keywordPlus | VASCULAR NICHE | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | OXYGEN | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | HYDROXYLATION | - |
dc.subject.keywordPlus | NEUROGENESIS | - |
dc.subject.keywordPlus | PLURIPOTENCY | - |
dc.subject.keywordAuthor | Microfluidic array | - |
dc.subject.keywordAuthor | Neural stem cells | - |
dc.subject.keywordAuthor | Hypoxia | - |
dc.subject.keywordAuthor | Self-renewal | - |
dc.subject.keywordAuthor | Differentiation | - |
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