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A microfluidic array for quantitative analysis of human neural stem cell self-renewal and differentiation in three-dimensional hypoxic microenvironment
- Authors
- Yang, Kisuk; Han, Sewoon; Shin, Yoojin; Ko, Eunkyung; Kim, Jin; Park, Kook In; Chung, Seok; Cho, Seung-Woo
- Issue Date
- 9월-2013
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Microfluidic array; Neural stem cells; Hypoxia; Self-renewal; Differentiation
- Citation
- BIOMATERIALS, v.34, no.28, pp.6607 - 6614
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOMATERIALS
- Volume
- 34
- Number
- 28
- Start Page
- 6607
- End Page
- 6614
- ISSN
- 0142-9612
- Abstract
- We report a microfluidic array for investigating and quantitatively analyzing human neural stem cell (hNSC) self-renewal and differentiation in an in vivo-like microenvironment. NSC niche conditions, including three-dimensional (3D) extracellular matrices and low oxygen tension, were effectively reconstituted in the microfluidic array in a combinatorial manner. The array device was fabricated to be detachable, rendering it compatible with quantitative real-time polymerase chain reaction for quantifying the effects of the biomimetic conditions on hNSC self-renewal and differentiation. We show that throughput of 3D cell culture and quantitative analysis can be increased. We also show that 3D hypoxic microenvironments maintain hNSC self-renewal capacity and direct neuronal commitment during hNSC differentiation. (C) 2013 Elsevier Ltd. All rights reserved.
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