Rapid Method for the Simultaneous Determination of Flavonol Aglycones in Food Using u-HPLC Coupled with Heating Block Acidic Hydrolysis
DC Field | Value | Language |
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dc.contributor.author | Shim, You-Shin | - |
dc.contributor.author | Kim, Seunghee | - |
dc.contributor.author | Seo, Dongwon | - |
dc.contributor.author | Ito, Masahito | - |
dc.contributor.author | Nakagawa, Hiroaki | - |
dc.contributor.author | Park, Hyun-Jin | - |
dc.contributor.author | Ha, Jaeho | - |
dc.date.accessioned | 2021-09-05T22:06:31Z | - |
dc.date.available | 2021-09-05T22:06:31Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2013-09 | - |
dc.identifier.issn | 1060-3271 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/102267 | - |
dc.description.abstract | A rapid method for the simultaneous determination of flavonol aglycones in food using ultra-high-performance LC (u-HPLC) coupled with a heating-block acidic hydrolysis method was validated in terms of precision, accuracy, and linearity. The u-HPLC separation was performed on an RP C18 column (particle size 2 inn id, 2 mm, length 100 mm) with a photodiode array detector. The LOD and LOQ of the u-HPLC analyses were 0.15 and 0.47 mg/kg for myricetin, 0.09 and 0.28 mg/kg for quercetin, 0.16 and 0.49 mg/kg for kaempferol, and 0.08 and 0.25 mg/kg for isorhamnetin. The intraday and interday precisions of the individual flavonol aglycones were less than 9.31%. All calibration curves exhibited good linearity (r(2) = 0.99) within the tested ranges. Total run time of u-HPLC was 13 min. The rapid u-HPLC method presented herein significantly improved the speed, sensitivity, and resolution of the analyses of myricetin, quercetin, kaempferol, and isorhamnetin in food. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AOAC INT | - |
dc.subject | PERFORMANCE LIQUID-CHROMATOGRAPHY | - |
dc.subject | ANTIOXIDANT ACTIVITIES | - |
dc.subject | IDENTIFICATION | - |
dc.subject | CULTIVARS | - |
dc.subject | QUANTIFICATION | - |
dc.subject | PROFILES | - |
dc.subject | LEAVES | - |
dc.subject | ANTHOCYANINS | - |
dc.subject | GLYCOSIDES | - |
dc.subject | QUERCETIN | - |
dc.title | Rapid Method for the Simultaneous Determination of Flavonol Aglycones in Food Using u-HPLC Coupled with Heating Block Acidic Hydrolysis | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Hyun-Jin | - |
dc.identifier.doi | 10.5740/jaoacint.12-439 | - |
dc.identifier.scopusid | 2-s2.0-84892187858 | - |
dc.identifier.wosid | 000326198600021 | - |
dc.identifier.bibliographicCitation | JOURNAL OF AOAC INTERNATIONAL, v.96, no.5, pp.1059 - 1064 | - |
dc.relation.isPartOf | JOURNAL OF AOAC INTERNATIONAL | - |
dc.citation.title | JOURNAL OF AOAC INTERNATIONAL | - |
dc.citation.volume | 96 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1059 | - |
dc.citation.endPage | 1064 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.subject.keywordPlus | PERFORMANCE LIQUID-CHROMATOGRAPHY | - |
dc.subject.keywordPlus | ANTIOXIDANT ACTIVITIES | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | CULTIVARS | - |
dc.subject.keywordPlus | QUANTIFICATION | - |
dc.subject.keywordPlus | PROFILES | - |
dc.subject.keywordPlus | LEAVES | - |
dc.subject.keywordPlus | ANTHOCYANINS | - |
dc.subject.keywordPlus | GLYCOSIDES | - |
dc.subject.keywordPlus | QUERCETIN | - |
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