Rapid Method for the Simultaneous Determination of Flavonol Aglycones in Food Using u-HPLC Coupled with Heating Block Acidic Hydrolysis
- Authors
- Shim, You-Shin; Kim, Seunghee; Seo, Dongwon; Ito, Masahito; Nakagawa, Hiroaki; Park, Hyun-Jin; Ha, Jaeho
- Issue Date
- 9월-2013
- Publisher
- AOAC INT
- Citation
- JOURNAL OF AOAC INTERNATIONAL, v.96, no.5, pp.1059 - 1064
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF AOAC INTERNATIONAL
- Volume
- 96
- Number
- 5
- Start Page
- 1059
- End Page
- 1064
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/102267
- DOI
- 10.5740/jaoacint.12-439
- ISSN
- 1060-3271
- Abstract
- A rapid method for the simultaneous determination of flavonol aglycones in food using ultra-high-performance LC (u-HPLC) coupled with a heating-block acidic hydrolysis method was validated in terms of precision, accuracy, and linearity. The u-HPLC separation was performed on an RP C18 column (particle size 2 inn id, 2 mm, length 100 mm) with a photodiode array detector. The LOD and LOQ of the u-HPLC analyses were 0.15 and 0.47 mg/kg for myricetin, 0.09 and 0.28 mg/kg for quercetin, 0.16 and 0.49 mg/kg for kaempferol, and 0.08 and 0.25 mg/kg for isorhamnetin. The intraday and interday precisions of the individual flavonol aglycones were less than 9.31%. All calibration curves exhibited good linearity (r(2) = 0.99) within the tested ranges. Total run time of u-HPLC was 13 min. The rapid u-HPLC method presented herein significantly improved the speed, sensitivity, and resolution of the analyses of myricetin, quercetin, kaempferol, and isorhamnetin in food.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Graduate School > Department of Biotechnology > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.