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Development of glycerol-utilizing Escherichia coli strain for the production of bioethanol
- Authors
- Thapa, Laxmi Prasad; Lee, Sang Jun; Yoo, Hah Young; Choi, Han Suk; Park, Chulhwan; Kim, Seung Wook
- Issue Date
- 15-8월-2013
- Publisher
- ELSEVIER SCIENCE INC
- Keywords
- Enterobacter aerogenes; Bioethanol production; Recombinant strains; Genetic manipulation
- Citation
- ENZYME AND MICROBIAL TECHNOLOGY, v.53, no.3, pp.206 - 215
- Indexed
- SCIE
SCOPUS
- Journal Title
- ENZYME AND MICROBIAL TECHNOLOGY
- Volume
- 53
- Number
- 3
- Start Page
- 206
- End Page
- 215
- ISSN
- 0141-0229
- Abstract
- The production of bioethanol was studied using recombinant Escherichia coli with glycerol as a carbon source. Glycerol is an attractive feedstock for biofuels production since it is generated as a major byproduct in biodiesel industry; therefore, we investigated the conversion of glycerol to bioethanol using E. coli BL21 (DE3) which harbors several genes in ethanol production pathway of Enterobacter aerogenes KCTC 2190. Fermentation was carried out at 34 degrees C for 42 h, pH 7.6, using defined production medium. Under optimal conditions, bioethanol production by the recombinant E. coli BL21 (DE3), strain pEB, was two-fold (3.01 g/L) greater than that (1.45 g/L) by the wild-type counterpart. The results obtained in this study will provide valuable guidelines for engineering bioethanol producers. (C) 2013 Elsevier Inc. All rights reserved.
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