Production, mechanical properties and in vitro biocompatibility of highly aligned porous poly(epsilon-caprolactone) (PCL)/hydroxyapatite (HA) scaffolds

Abstract

We produced highly aligned porous poly(epsilon-caprolactone) (PCL)/hydroxyapatite (HA) scaffolds by unidirectionally freezing PCL/HA solutions with various HA contents (0, 5, 10 and 20 wt% in relation to the PCL polymer) and evaluated their mechanical properties and in vitro biocompatibility to examine their potential applications in bone tissue engineering. All the prepared scaffolds had a highly aligned porous structure, in which the HA particles were uniformly dispersed in the PCL walls. The elastic modulus of the PCL/HA scaffolds significantly increased from 0.12 +/- A 0.02 to 2.65 +/- A 0.05 MPa with increasing initial HA content from 0 to 20 wt%, whereas the pore size decreased from 9.2 +/- A 0.7 to 4.2 +/- A 0.8 mu m. In addition, the PCL/HA scaffolds showed considerably enhanced in vitro cellular responses that were assessed in terms of cell attachment, proliferation and osteoblastic differentiation.