Detailed Information
Cited 0 time in 
Cited 0 time in 
Cited 0 time in
Metadata Downloads
A Self-Calibrating Bipartite Viscosity Sensor for Mitochondria
- Authors
- Yang, Zhigang; He, Yanxia; Lee, Jae-Hong; Park, Nayoung; Suh, Myungkoo; Chae, Weon-Sik; Cao, Jianfang; Peng, Xiaojun; Jung, Hyosung; Kang, Chulhun; Kim, Jong Seung
- Issue Date
- 19-6월-2013
- Publisher
- AMER CHEMICAL SOC
- Citation
- JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, v.135, no.24, pp.9181 - 9185
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
- Volume
- 135
- Number
- 24
- Start Page
- 9181
- End Page
- 9185
- ISSN
- 0002-7863
- Abstract
- A self-calibrating bipartite viscosity sensor 1 for cellular mitochondria, composed of coumarin and boron-dipyrromethene (BODIPY) with a rigid phenyl spacer and a mitochondria-targeting unit, was synthesized. The sensor showed a direct linear relationship between the fluorescence intensity ratio of BODIPY to coumarin or the fluorescence lifetime ratio and the media viscosity, which allowed us to determine the average mitochondrial viscosity in living HeLa cells as ca. 62 cP (cp). Upon treatment with an ionophore, monensin, or nystatin, the mitochondrial viscosity was observed to increase to ca. 110 cP.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Science > Department of Chemistry > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.