Fluorogenic pH-Sensitive Polydiacetylene (PDA) Liposomes as a Drug Carrier
- Authors
- Won, Sang Ho; Lee, Jong Uk; Sim, Sang Jun
- Issue Date
- 6월-2013
- Publisher
- AMER SCIENTIFIC PUBLISHERS
- Keywords
- Polydiacetylene; Liposome; Fluorescence; Drug Carrier; pH-Sensitive; Ampicillin
- Citation
- JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.13, no.6, pp.3792 - 3800
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
- Volume
- 13
- Number
- 6
- Start Page
- 3792
- End Page
- 3800
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/103031
- DOI
- 10.1166/jnn.2013.7205
- ISSN
- 1533-4880
- Abstract
- A crucial issue for current liposomal carriers in clinical applications is the sustained-release property of the encapsulated drugs. We have developed novel fluorogenic pH-sensitive polymerized liposomes composed of polydiacetylene (PDA) lipids and other types of lipids. Unilamellar liposomes containing 10,12-pentacosadiynoic acid (PCDA), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), and N-palmitoyl homocysteine (PHC) were loaded with ampicillin. These liposomes fused to each other rapidly when the medium pH was lowered from 7 to 4. The polymerized liposomes were characterized in terms of particle size distribution. The liposome size increased approximately 20-fold from 110.0 +/- 19.3 nm to 2046.7 +/- 487.4 nm as the pH was lowered. Cross-linking of the diacetylene lipids prevents drug leakage and the encapsulated drug can be instantaneously released at acidic pH condition. The ampicillin was nearly completely released (74.4 +/- 3.9%) from liposomes within 4 h under acidic pH conditions and the released amounts of ampicillin were analyzed by HPLC. Finally, the therapeutic effect was observed by the appearance of plaques on a lawn of E. coli, and fluorescent images of the PDA liposomes were taken from the plaques for drug release monitoring. As a result, this research demonstrates that such novel pH-sensitive polymerized liposomes have great prospects as a drug carrier.
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Collections - College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles
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