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An ammonium sulfate sensitive endoxylanase produced by Streptomyces

Authors
Simkhada, Jaya RamYoo, Hah YoungPark, Don HeeChoi, Yun HeeLee, Hyo JeongKim, Seung WookYoo, Jin Cheol
Issue Date
6월-2013
Publisher
SPRINGER
Keywords
Xylanase; Streptomyces sp CSWu2; Agro-industrial biomass; Ion exchange chromatography; Corncob
Citation
BIOPROCESS AND BIOSYSTEMS ENGINEERING, v.36, no.6, pp.819 - 825
Indexed
SCIE
SCOPUS
Journal Title
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Volume
36
Number
6
Start Page
819
End Page
825
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/103171
DOI
10.1007/s00449-013-0908-4
ISSN
1615-7591
Abstract
Streptomyces sp. CSWu2 was newly isolated and identified from Korean soil. In culture medium, the strain produced a highly active endoxylanase (Xynwu2), which was purified to homogeneity by a single-step chromatography on Poros-HQ. The xylanase was similar to 38 kDa and its activity was maximal at 65 degrees C and pH 11.0. It was stable up to 60 degrees C and from pH 8.0 to 12.0, and its activity was slightly enhanced by nonionic detergents, but inhibited by EDTA, EGTA, and divalent metal ions. Intriguingly, Xynwu2 was highly sensitive to ammonium sulfate, but its completely suppressed activity was recovered by desalting out. Xynwu2 produced xylose and xylobiose as principal end products from xylan, suggesting an endoxylanase nature. Importantly, scanning electron microscopy showed Xynwu2 efficiently degraded corncobs, an agro-industrial waste material. We believe that Xynwu2 is a potential candidate for converting lignocellulosic waste material into simple sugars which could be used to produce bioethanol and other value-added products.
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