The effect of oxidized low-density lipoprotein (ox-LDL) on radiation-induced endothelial-to-mesenchymal transition
- Authors
- Kim, Miseon; Choi, Seo-Hyun; Jin, Yeung Bae; Lee, Hae-June; Ji, Young Hoon; Kim, Joon; Lee, Yun-Sil; Lee, Yoon-Jin
- Issue Date
- 5월-2013
- Publisher
- INFORMA HEALTHCARE
- Keywords
- Atherosclerosis; radiation; endothelial-to-mesenchymal transition (EndMT); oxidized low-density lipoprotein (ox-LDL)
- Citation
- INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, v.89, no.5, pp.356 - 363
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
- Volume
- 89
- Number
- 5
- Start Page
- 356
- End Page
- 363
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/103370
- DOI
- 10.3109/09553002.2013.763193
- ISSN
- 0955-3002
- Abstract
- Purpose : Radiation-induced cardiovascular disease is a potentially severe side-effect of thoracic radiotherapy treatment. Clinically, this delayed side-effect presents as a form of accelerated atherosclerosis several years after irradiation. As general endothelial dysfunction is known to be an initiating event in radiation-induced vascular damage, we examined the effects of radiation on endothelial cells in radiation-induced atherosclerosis. Materials and methods : The effects of radiation on human aortic endothelial cells (HAoEC) were assessed by immunoblotting and immunofluorescence assays. Radiation-induced phenotypic changes of endothelial cells (ECs) were examined using atherosclerotic tissues of irradiated apoprotein E null (ApoE(-/-)) mice. Results : Radiation induced the HAoEC to undergo phenotypic conversion to form fibroblast-like cells, called the endothelial-to-mesenchymal transition (EndMT), which leads to the upregulation of mesenchymal cell markers such as alpha-smooth muscle actin (alpha-SMA), fibroblast specific protein-1 (FSP-1), and vimentin, and downregulation of endothelial cell-specific markers such as CD31 and vascular endothelial (VE)-cadherin. Furthermore, compared with low-density lipoprotein (LDL), oxidized low-density lipoprotein (ox-LDL) significantly augmented radiation-induced EndMT in HAoEC. These fibrotic phenotypes of ECs were found in atherosclerotic tissues of irradiated ApoE(-/-) mice with increased levels of ox-LDL. Conclusions : Taken together, these observations suggest that ox-LDL accelerates radiation-induced EndMT and subsequently contributes to radiation-induced atherosclerosis, providing a novel target for the prevention of radiation-induced atherosclerosis.
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Collections - Graduate School > Department of Life Sciences > 1. Journal Articles
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