Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Improvement of the Diagnostic Sensitivity of Scrub Typhus Using a Mixture of Recombinant Antigens Derived from Orientia tsutsugamushi Serotypes

Authors
Kim, Young-JinYeo, Seon-JuPark, Sang-JinWoo, Ye-JuKim, Min-WooKim, Seung-HanChang, In-AeJeon, Sung-HoPark, Byung-JaeSong, Gi-JunLee, Myoung-GuKim, Ik-SangKim, Yoon-Won
Issue Date
5월-2013
Publisher
KOREAN ACAD MEDICAL SCIENCES
Keywords
Scrub Typhus; Orientia tsutsugamushi; Recombinant Antigen; IFA; ELISA; RDT
Citation
JOURNAL OF KOREAN MEDICAL SCIENCE, v.28, no.5, pp.672 - 679
Indexed
SCIE
SCOPUS
KCI
Journal Title
JOURNAL OF KOREAN MEDICAL SCIENCE
Volume
28
Number
5
Start Page
672
End Page
679
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/103420
DOI
10.3346/jkms.2013.28.5.672
ISSN
1011-8934
Abstract
Diagnosis of scrub typhus is difficult because its symptoms are very similar to other acute febrile illnesses, such as leptospirosis, murine typhus, and other viral hemorrhagic fevers. To differentiate scrub typhus from other acute febrile diseases, a rapid and reliable serological diagnosis is important. We have developed a chimeric recombinant antigen cr56 and two other recombinant antigens, r21 and kr56, from various serotypes of Orientia tsutsugamushi. They were tested for the detection of antibodies against O. tsutsugamushi in the patient's serum samples using enzyme-linked immunosorbent assay (ELISA) and dot-blot analyses. As of conventional immunofluorescence assay (IFA), when the mixture of these three recombinant antigens was used, both sensitivity and specificity of the recombinant antigens were increased up to 98% in IgM and IgG at ELISA and dot blotting. Additionally, both sensitivity and specificity by detection of IgM and IgG antibodies at rapid diagnostic test (RDT), using the mixture of three antigens and gold conjugated antibodies, were 99%. Our results suggest the use of mixture of these recombinant antigen proteins in ELISA or RDT is suitable as a diagnostic test for scrub typhus.
Files in This Item
There are no files associated with this item.
Appears in
Collections
College of Medicine > Department of Medical Science > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Altmetrics

Total Views & Downloads

BROWSE