Enzymatic fuel cells based on electrodeposited graphite oxide/cobalt hydroxide/chitosan composite-enzyme electrode

Abstract

Enzymatic fuel cells (EFCs) use redox enzymes with high electron transfer rates that lead to high power density from bioavailable substrates. However, EFCs are limited by the difficult electrical wiring of the enzymes to the electrode. Therefore, deposition of Co(OH)(2) onto graphite oxide (GO) was improved for efficient wiring of the enzymes. The GO/Co(OH)(2)/chitosan composites were electrodeposited for immobilization of glucose oxidase (GOD) or laccase on an Au electrode, respectively. The electrical properties of the bioelectrode according to cyclic voltammetry were improved using GO/Co(OH)(2)/chitosan composites. The anode and cathode system was composed of GOD and laccase as biocatalysts and glucose/oxygen as substrates under ambient conditions (pH 7.0 and 25 degrees C). The EFC using GO/Co(OH)(2)/chitosan composites with a mediator delivered a high power density of up to 517 +/- 3.3 mu W/cm(2) at 0.46 V and open circuit voltage of 0.60 V. These results provide a promising direction for further development and application of EFCs. (c) 2012 Elsevier B.V. All rights reserved.