Size-controllable networked neurospheres as a 3D neuronal tissue model for Alzheimer's disease studies
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Yoon Jung | - |
dc.contributor.author | Park, JiSoo | - |
dc.contributor.author | Lee, Sang-Hoon | - |
dc.date.accessioned | 2021-09-06T03:11:11Z | - |
dc.date.available | 2021-09-06T03:11:11Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2013-04 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/103667 | - |
dc.description.abstract | Intensive in vitro studies on the neurotoxicity of amyloid beta have been conducted for decades; however, a three-dimensional neuronal tissue model for Alzheimer's disease has not yet been achieved. In this study, we developed size-controllable networked neurospheres comprised of cerebral cortical neuronal cells that mimics the cytoarchitecture of the cortical region of the brain. The toxicity of amyloid beta on the neurosphere model was assessed quantitatively and qualitatively. Decreased cell viability after amyloid beta exposure was demonstrated using MIT and live/dead assays. Neurite degeneration after amyloid beta exposure was evident in both SEM and fluorescence images. Ultrastructural features of apoptotic neurons were analyzed and quantitative analysis of synapsin II concentration and an acetylcholine assay were also performed. The three-dimensional neurospheres, produced using a concave microwell array, are a potential in vitro model for Alzheimer's disease studies. (C) 2013 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | CEREBRAL-CORTEX | - |
dc.subject | NEURAL TISSUE | - |
dc.subject | APOPTOSIS | - |
dc.subject | CELLS | - |
dc.subject | ONSET | - |
dc.title | Size-controllable networked neurospheres as a 3D neuronal tissue model for Alzheimer's disease studies | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Sang-Hoon | - |
dc.identifier.doi | 10.1016/j.biomaterials.2013.01.038 | - |
dc.identifier.scopusid | 2-s2.0-84873476770 | - |
dc.identifier.wosid | 000316038900009 | - |
dc.identifier.bibliographicCitation | BIOMATERIALS, v.34, no.12, pp.2938 - 2946 | - |
dc.relation.isPartOf | BIOMATERIALS | - |
dc.citation.title | BIOMATERIALS | - |
dc.citation.volume | 34 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 2938 | - |
dc.citation.endPage | 2946 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | CEREBRAL-CORTEX | - |
dc.subject.keywordPlus | NEURAL TISSUE | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | ONSET | - |
dc.subject.keywordAuthor | Neurosphere | - |
dc.subject.keywordAuthor | 3D model | - |
dc.subject.keywordAuthor | Amyloid beta | - |
dc.subject.keywordAuthor | Alzheimer&apos | - |
dc.subject.keywordAuthor | s disease | - |
dc.subject.keywordAuthor | PDMS microconcave wells | - |
dc.subject.keywordAuthor | Neural networks | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.