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Size-controllable networked neurospheres as a 3D neuronal tissue model for Alzheimer's disease studies

Authors
Choi, Yoon JungPark, JiSooLee, Sang-Hoon
Issue Date
4월-2013
Publisher
ELSEVIER SCI LTD
Keywords
Neurosphere; 3D model; Amyloid beta; Alzheimer' s disease; PDMS microconcave wells; Neural networks
Citation
BIOMATERIALS, v.34, no.12, pp.2938 - 2946
Indexed
SCIE
SCOPUS
Journal Title
BIOMATERIALS
Volume
34
Number
12
Start Page
2938
End Page
2946
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/103667
DOI
10.1016/j.biomaterials.2013.01.038
ISSN
0142-9612
Abstract
Intensive in vitro studies on the neurotoxicity of amyloid beta have been conducted for decades; however, a three-dimensional neuronal tissue model for Alzheimer's disease has not yet been achieved. In this study, we developed size-controllable networked neurospheres comprised of cerebral cortical neuronal cells that mimics the cytoarchitecture of the cortical region of the brain. The toxicity of amyloid beta on the neurosphere model was assessed quantitatively and qualitatively. Decreased cell viability after amyloid beta exposure was demonstrated using MIT and live/dead assays. Neurite degeneration after amyloid beta exposure was evident in both SEM and fluorescence images. Ultrastructural features of apoptotic neurons were analyzed and quantitative analysis of synapsin II concentration and an acetylcholine assay were also performed. The three-dimensional neurospheres, produced using a concave microwell array, are a potential in vitro model for Alzheimer's disease studies. (C) 2013 Elsevier Ltd. All rights reserved.
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