Detection of VR-2332 Strain of Porcine Reproductive and Respiratory Syndrome Virus Type II Using an Aptamer-Based Sandwich-Type Assay
DC Field | Value | Language |
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dc.contributor.author | Lee, Su Jin | - |
dc.contributor.author | Kwon, Young Seop | - |
dc.contributor.author | Lee, Ji-eun | - |
dc.contributor.author | Choi, Eun-Jin | - |
dc.contributor.author | Lee, Chang-Hee | - |
dc.contributor.author | Song, Jae-Young | - |
dc.contributor.author | Gu, Man Bock | - |
dc.date.accessioned | 2021-09-06T05:29:58Z | - |
dc.date.available | 2021-09-06T05:29:58Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2013-01-01 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/104240 | - |
dc.description.abstract | Porcine reproductive and respiratory syndrome virus (PRRSV) causes porcine reproductive and respiratory syndrome disease (PRRS), a disease that has a significant and economic impact on the swine industry. In this study, single-stranded DNA (ssDNA) aptamers with high specificity and affinity against VR-2332 strain of PRRSV type II were successfully obtained. Of 19 candidates, the LB32 aptamer was found to be the most specific and sensitive to VR-2332 strain according to an aptamer-based surface plasmon resonance (SPR) analysis. The detection of VR-2332 of PRRSV type II was successfully accomplished using the enzyme-linked antibody-aptamer sandwich (ELAAS) method. The detection limit of ELAAS was 4.8 X 10(0) TCID50/mL that is comparable to some of the previous reports of the PCR-based detection but does not require any complicated equipment or extra costs. Moreover, this ELAAS-based PRRSV detection showed similar sensitivity for both the VR-2332 samples spiked in diluted swine serum and in buffer. Therefore, this VR-2332 strain-specific aptamer and its assay method with high specificity can be used as an alternative method for the fast and precise detection of PRRSV. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | RNA APTAMER | - |
dc.subject | BOAR SEMEN | - |
dc.subject | NORTH-AMERICAN | - |
dc.subject | IN-VITRO | - |
dc.subject | RT-PCR | - |
dc.subject | TRANSCRIPTION | - |
dc.subject | PRRSV | - |
dc.subject | HEMAGGLUTININ | - |
dc.subject | DIAGNOSIS | - |
dc.subject | DIFFERENTIATION | - |
dc.title | Detection of VR-2332 Strain of Porcine Reproductive and Respiratory Syndrome Virus Type II Using an Aptamer-Based Sandwich-Type Assay | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Gu, Man Bock | - |
dc.identifier.doi | 10.1021/ac3026866 | - |
dc.identifier.scopusid | 2-s2.0-84871736815 | - |
dc.identifier.wosid | 000313156500013 | - |
dc.identifier.bibliographicCitation | ANALYTICAL CHEMISTRY, v.85, no.1, pp.66 - 74 | - |
dc.relation.isPartOf | ANALYTICAL CHEMISTRY | - |
dc.citation.title | ANALYTICAL CHEMISTRY | - |
dc.citation.volume | 85 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 66 | - |
dc.citation.endPage | 74 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | RNA APTAMER | - |
dc.subject.keywordPlus | BOAR SEMEN | - |
dc.subject.keywordPlus | NORTH-AMERICAN | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | RT-PCR | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
dc.subject.keywordPlus | PRRSV | - |
dc.subject.keywordPlus | HEMAGGLUTININ | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
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