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Detection of VR-2332 Strain of Porcine Reproductive and Respiratory Syndrome Virus Type II Using an Aptamer-Based Sandwich-Type Assay

Authors
Lee, Su JinKwon, Young SeopLee, Ji-eunChoi, Eun-JinLee, Chang-HeeSong, Jae-YoungGu, Man Bock
Issue Date
1-1월-2013
Publisher
AMER CHEMICAL SOC
Citation
ANALYTICAL CHEMISTRY, v.85, no.1, pp.66 - 74
Indexed
SCIE
SCOPUS
Journal Title
ANALYTICAL CHEMISTRY
Volume
85
Number
1
Start Page
66
End Page
74
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/104240
DOI
10.1021/ac3026866
ISSN
0003-2700
Abstract
Porcine reproductive and respiratory syndrome virus (PRRSV) causes porcine reproductive and respiratory syndrome disease (PRRS), a disease that has a significant and economic impact on the swine industry. In this study, single-stranded DNA (ssDNA) aptamers with high specificity and affinity against VR-2332 strain of PRRSV type II were successfully obtained. Of 19 candidates, the LB32 aptamer was found to be the most specific and sensitive to VR-2332 strain according to an aptamer-based surface plasmon resonance (SPR) analysis. The detection of VR-2332 of PRRSV type II was successfully accomplished using the enzyme-linked antibody-aptamer sandwich (ELAAS) method. The detection limit of ELAAS was 4.8 X 10(0) TCID50/mL that is comparable to some of the previous reports of the PCR-based detection but does not require any complicated equipment or extra costs. Moreover, this ELAAS-based PRRSV detection showed similar sensitivity for both the VR-2332 samples spiked in diluted swine serum and in buffer. Therefore, this VR-2332 strain-specific aptamer and its assay method with high specificity can be used as an alternative method for the fast and precise detection of PRRSV.
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