Molecular characterization of the AtCXE8 gene, which promotes resistance to Botrytis cinerea infection
DC Field | Value | Language |
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dc.contributor.author | Lee, Sanghyeob | - |
dc.contributor.author | Hwang, Seongbin | - |
dc.contributor.author | Seo, Yong Weon | - |
dc.contributor.author | Jeon, Woong Bae | - |
dc.contributor.author | Oh, Boung-Jun | - |
dc.date.accessioned | 2021-09-06T05:48:25Z | - |
dc.date.available | 2021-09-06T05:48:25Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2013-01 | - |
dc.identifier.issn | 1863-5466 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/104349 | - |
dc.description.abstract | The relationships of sterase- and lipase-like proteins with three signaling molecules (salicylic acid, jasmonic acid, and ethylene) expressed during plant-pathogen interactions were studied. We isolated two carboxylesterase (AtCXE) genes, AtCXE8 and AtCXE9, from Arabidopsis thaliana. The AtCXE8 and AtCXE9 proteins possess carboxylesterase motifs (-GXSXG-) and catalytic triads (Ser, Asp, and His). We demonstrated that recombinant AtCXE8 and AtCXE9 proteins have both enzymatic activity and specific activity for p-nitrophenyl butyrate (C-4) in vitro. Moreover, the enzymatic activity of recombinant AtCXE8 was twofold higher than that of AtCXE9. To gain a better understanding of the endogenous role of the AtCXE8 gene in Arabidopsis, we identified an enhancer trap T-DNA mutant (AtCXE8_KO) and used it to show that the AtCXE8 gene was induced in response to fungal infection. AtCXE8_KO plants were also more susceptible to infections than wild-type Col-0 plants. Moreover, overexpression of the AtCXE8 gene in transgenic Arabidopsis plants led to enhanced disease resistance against B. cinerea. Taken together, our data indicate that AtCXE8 plays a role in promoting resistance to fungal invasion. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.subject | SYSTEMIC ACQUIRED-RESISTANCE | - |
dc.subject | PLANT DEFENSIN GENE | - |
dc.subject | SALICYLIC-ACID | - |
dc.subject | ARABIDOPSIS-THALIANA | - |
dc.subject | DEFICIENT MUTANTS | - |
dc.subject | RESPONSE PATHWAYS | - |
dc.subject | MORAXELLA TA144 | - |
dc.subject | JASMONIC ACID | - |
dc.subject | PAD4 ENCODES | - |
dc.subject | ETHYLENE | - |
dc.title | Molecular characterization of the AtCXE8 gene, which promotes resistance to Botrytis cinerea infection | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Seo, Yong Weon | - |
dc.identifier.doi | 10.1007/s11816-012-0253-0 | - |
dc.identifier.scopusid | 2-s2.0-84872319154 | - |
dc.identifier.wosid | 000313416600011 | - |
dc.identifier.bibliographicCitation | PLANT BIOTECHNOLOGY REPORTS, v.7, no.1, pp.109 - 119 | - |
dc.relation.isPartOf | PLANT BIOTECHNOLOGY REPORTS | - |
dc.citation.title | PLANT BIOTECHNOLOGY REPORTS | - |
dc.citation.volume | 7 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 109 | - |
dc.citation.endPage | 119 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001848906 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.subject.keywordPlus | SYSTEMIC ACQUIRED-RESISTANCE | - |
dc.subject.keywordPlus | PLANT DEFENSIN GENE | - |
dc.subject.keywordPlus | SALICYLIC-ACID | - |
dc.subject.keywordPlus | ARABIDOPSIS-THALIANA | - |
dc.subject.keywordPlus | DEFICIENT MUTANTS | - |
dc.subject.keywordPlus | RESPONSE PATHWAYS | - |
dc.subject.keywordPlus | MORAXELLA TA144 | - |
dc.subject.keywordPlus | JASMONIC ACID | - |
dc.subject.keywordPlus | PAD4 ENCODES | - |
dc.subject.keywordPlus | ETHYLENE | - |
dc.subject.keywordAuthor | Abiotic stress | - |
dc.subject.keywordAuthor | Botrytis cinerea | - |
dc.subject.keywordAuthor | Carboxylesterase | - |
dc.subject.keywordAuthor | Disease resistance | - |
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