Molecular characterization of the AtCXE8 gene, which promotes resistance to Botrytis cinerea infection
- Authors
- Lee, Sanghyeob; Hwang, Seongbin; Seo, Yong Weon; Jeon, Woong Bae; Oh, Boung-Jun
- Issue Date
- 1월-2013
- Publisher
- SPRINGER
- Keywords
- Abiotic stress; Botrytis cinerea; Carboxylesterase; Disease resistance
- Citation
- PLANT BIOTECHNOLOGY REPORTS, v.7, no.1, pp.109 - 119
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- PLANT BIOTECHNOLOGY REPORTS
- Volume
- 7
- Number
- 1
- Start Page
- 109
- End Page
- 119
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/104349
- DOI
- 10.1007/s11816-012-0253-0
- ISSN
- 1863-5466
- Abstract
- The relationships of sterase- and lipase-like proteins with three signaling molecules (salicylic acid, jasmonic acid, and ethylene) expressed during plant-pathogen interactions were studied. We isolated two carboxylesterase (AtCXE) genes, AtCXE8 and AtCXE9, from Arabidopsis thaliana. The AtCXE8 and AtCXE9 proteins possess carboxylesterase motifs (-GXSXG-) and catalytic triads (Ser, Asp, and His). We demonstrated that recombinant AtCXE8 and AtCXE9 proteins have both enzymatic activity and specific activity for p-nitrophenyl butyrate (C-4) in vitro. Moreover, the enzymatic activity of recombinant AtCXE8 was twofold higher than that of AtCXE9. To gain a better understanding of the endogenous role of the AtCXE8 gene in Arabidopsis, we identified an enhancer trap T-DNA mutant (AtCXE8_KO) and used it to show that the AtCXE8 gene was induced in response to fungal infection. AtCXE8_KO plants were also more susceptible to infections than wild-type Col-0 plants. Moreover, overexpression of the AtCXE8 gene in transgenic Arabidopsis plants led to enhanced disease resistance against B. cinerea. Taken together, our data indicate that AtCXE8 plays a role in promoting resistance to fungal invasion.
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Collections - Graduate School > Department of Plant Biotechnology > 1. Journal Articles
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