Morphological Changes of Human Mesenchymal Stem Cells on a Large Area of Nanopatterned Surface
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이재연 | - |
dc.contributor.author | 이규백 | - |
dc.contributor.author | 선경 | - |
dc.contributor.author | 박용두 | - |
dc.date.accessioned | 2021-09-06T07:14:11Z | - |
dc.date.available | 2021-09-06T07:14:11Z | - |
dc.date.created | 2021-06-17 | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 1226-4601 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/104930 | - |
dc.description.abstract | Cells receive various physical and chemical signals from their tissue’s microenvironment. In this study, human mesenchymal stem cells were cultured on variously sized nanostructures and their cellular responses were evaluated. rious nanotopological structures were fabricated on polystyrene polymer surfaces using anodized aluminum oxide (AAO) with different nanopost diameters (70, 200, 400 nm) as nanoimprinting templates. Before cell culture experiments,nanopatterns were coated with collagen types I and IV. Human mesenchymal stem cells were cultured on the various nanotopological structures for 24 h. Cell viability in the nanopatterned surfaces was evaluated by a live/dead assay which showed that various nanostructures do not affect cellular viability. Cellular viability in all the samples was more than 95%. Cell proliferation assays showed that cells cultured on 70 nm nanostructures pre-coated with collagen type IV increased proliferation rates three folds compared to those of control, 200 nm, or 400 nm surfaces. Additionally, the cell area showed significant differences between samples cultured on 70 nm nanopatterned pre-coated with collagen type IV surfaces compared to other conditions. However, Nanopatterned surfaces pre-coated with collagen type I showed no significant differences between samples cultured on a 70 nm nanopatterned surface compared to the other samples. This result reflects that cells showed differential reaction depending on surface coated ECM proteins as well as nano structures. The effect of pre-coated ECM proteins is one of the major determinants that guides cellular reactions and nanopatterned surfaces could be used to further guide stem cell differentiation and proliferation. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | 한국생체재료학회 | - |
dc.title | Morphological Changes of Human Mesenchymal Stem Cells on a Large Area of Nanopatterned Surface | - |
dc.title.alternative | Morphological Changes of Human Mesenchymal Stem Cells on a Large Area of Nanopatterned Surface | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | 박용두 | - |
dc.identifier.bibliographicCitation | 생체재료학회지, v.17, no.1, pp.1 - 6 | - |
dc.relation.isPartOf | 생체재료학회지 | - |
dc.citation.title | 생체재료학회지 | - |
dc.citation.volume | 17 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 6 | - |
dc.type.rims | ART | - |
dc.identifier.kciid | ART001746989 | - |
dc.description.journalClass | 2 | - |
dc.description.journalRegisteredClass | kci | - |
dc.subject.keywordAuthor | extracellular matrix | - |
dc.subject.keywordAuthor | collagen | - |
dc.subject.keywordAuthor | nanotopography | - |
dc.subject.keywordAuthor | hMSC | - |
dc.subject.keywordAuthor | cell area | - |
dc.subject.keywordAuthor | proliferation | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.