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Morphological Changes of Human Mesenchymal Stem Cells on a Large Area of Nanopatterned SurfaceMorphological Changes of Human Mesenchymal Stem Cells on a Large Area of Nanopatterned Surface

Other Titles
Morphological Changes of Human Mesenchymal Stem Cells on a Large Area of Nanopatterned Surface
Authors
이재연이규백선경박용두
Issue Date
2013
Publisher
한국생체재료학회
Keywords
extracellular matrix; collagen; nanotopography; hMSC; cell area; proliferation
Citation
생체재료학회지, v.17, no.1, pp.1 - 6
Indexed
KCI
Journal Title
생체재료학회지
Volume
17
Number
1
Start Page
1
End Page
6
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/104930
ISSN
1226-4601
Abstract
Cells receive various physical and chemical signals from their tissue’s microenvironment. In this study, human mesenchymal stem cells were cultured on variously sized nanostructures and their cellular responses were evaluated. rious nanotopological structures were fabricated on polystyrene polymer surfaces using anodized aluminum oxide (AAO) with different nanopost diameters (70, 200, 400 nm) as nanoimprinting templates. Before cell culture experiments,nanopatterns were coated with collagen types I and IV. Human mesenchymal stem cells were cultured on the various nanotopological structures for 24 h. Cell viability in the nanopatterned surfaces was evaluated by a live/dead assay which showed that various nanostructures do not affect cellular viability. Cellular viability in all the samples was more than 95%. Cell proliferation assays showed that cells cultured on 70 nm nanostructures pre-coated with collagen type IV increased proliferation rates three folds compared to those of control, 200 nm, or 400 nm surfaces. Additionally, the cell area showed significant differences between samples cultured on 70 nm nanopatterned pre-coated with collagen type IV surfaces compared to other conditions. However, Nanopatterned surfaces pre-coated with collagen type I showed no significant differences between samples cultured on a 70 nm nanopatterned surface compared to the other samples. This result reflects that cells showed differential reaction depending on surface coated ECM proteins as well as nano structures. The effect of pre-coated ECM proteins is one of the major determinants that guides cellular reactions and nanopatterned surfaces could be used to further guide stem cell differentiation and proliferation.
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