Effects of 17β-estradiol and estrogen receptor antagonists on the proliferation of gastric cancer cell lines
- Authors
- Kim, M.-J.; Cho, S.-I.; Lee, K.-O.; Han, H.-J.; Song, T.-J.; Park, S.-H.
- Issue Date
- 2013
- Keywords
- Cell line; Estrogen; Estrogens; Receptors; Stomach neoplasms
- Citation
- Journal of Gastric Cancer, v.13, no.3, pp.172 - 178
- Indexed
- SCOPUS
KCI
OTHER
- Journal Title
- Journal of Gastric Cancer
- Volume
- 13
- Number
- 3
- Start Page
- 172
- End Page
- 178
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/105970
- DOI
- 10.5230/jgc.2013.13.3.172
- ISSN
- 2093-582X
- Abstract
- Purpose: The aims of this study were as follow: 1) to describe the expression status of estrogen receptor-α and-β mRNAs in five gastric carcinoma cell lines; 2) to evaluate in vitro the effects of 17β-estradiol and estrogen receptor antagonists on the proliferation of the cell lines. Materials and Methods: Detection of estrogen receptor-α and estrogen receptor-β mRNA in five human gastric cancer cell lines (AGS, KATO III, MKN28, MKN45 and MKN74) was made by the reverse transcription-polymerase chain reaction system. To evaluate the effect of 17β-estradiol and estrogen receptor antagonists on the proliferation of gastric cancer cell line, the cell lines which expressed both estrogen receptors were chosen and treated with 17β-estradiol and estrogen receptor antagonists (methyl-piperidino-pyrazole and pyrazolo [1,5-a] pyrimidine). Cell proliferation was assessed with the methylthiazol tetrazolium test. Results: Estrogen receptor-α and estrogen receptor-β mRNAs were expressed in three (KATO III, MKN28 and MKN45) and all of the five gastric cancer cell lines, respectively. At higher concentrations, 17β-estradiol inhibited cell growth of MKN28, MKN45 and KATO III cell lines. Neither estrogen receptor-α nor estrogen receptor-β antagonist blocked the anti-proliferative effect of 17β-estradiol. Conclusions: Our results indicate that estrogen receptor-β mRNAs are preferentially expressed in gastric cancers and also imply that hormone therapy rather than estrogen receptor blockers may be a useful strategy for the treatment of estrogen receptor-β positive gastric cancer. Its therapeutic significance in gastric cancer are, however, limited until more evidence of the roles of estrogen receptors in the gastric cancer are accumulated. © 2013 by The Korean Gastric Cancer Association.
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