Acyl-CoA thioesterase 8 is a specific protein related to nodal metastasis and prognosis of lung adenocarcinoma
- Authors
- Jung, Woon Yong; Kim, Young Hye; Ryu, Young Joon; Kim, Baek-Hui; Shin, Bong Kyung; Kim, Aeree; Kim, Han Kyeom
- Issue Date
- 2013
- Publisher
- ELSEVIER GMBH
- Keywords
- Lung; Adenocarcinoma; Neoplasm metastasis; Prognosis; Proteomics
- Citation
- PATHOLOGY RESEARCH AND PRACTICE, v.209, no.5, pp.276 - 283
- Indexed
- SCIE
SCOPUS
- Journal Title
- PATHOLOGY RESEARCH AND PRACTICE
- Volume
- 209
- Number
- 5
- Start Page
- 276
- End Page
- 283
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/106537
- DOI
- 10.1016/j.prp.2013.02.008
- ISSN
- 0344-0338
- Abstract
- Metastasis is a major cause of cancer recurrence or death. This study attempted to quantitatively identify different proteins in metastatic lung adenocarcinoma. The NIT quotient [number of metastatic lymph nodes (n)/tumor diameter (cm)] was used to select samples with an extreme metastatic phenotype. Among the six fresh frozen lung adenocarcinoma specimens, the three showing the highest NIT quotient represented the metastatic group, and others with the greatest tumor diameters without metastasis represented the non-metastatic group. After 2-dimensional electrophoresis, the significantly different protein spots were selected by image analysis and analyzed with MALDI-TOF mass spectrometry. Acyl-CoA thioesterase 8 isoform c (ACOT8) was one of most overexpressed proteins in the metastatic group, and it was validated by Western blot and immunohistochemical staining on 108 paraffin-embedded tumor samples. High ACOT8 expression was correlated with lymph node metastasis (p = 0.002), recurrence (p = 0.034), predominant histologic subtypes (p = 0.007), and higher stage (p = 0.005). In multivariate analysis, high ACOT8 expression was significantly associated with increased risks of lymph node metastasis (p = 0.009) and cancer-related death (p = 0.030), independent of clinical factors. ACOT8 may be a candidate prognostic biomarker and therapeutic target of lung adenocarcinoma. (C) 2013 Elsevier GmbH. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
- Graduate School > Department of Biomedical Sciences > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.