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Comprehensive and high-resolution typing of swine leukocyte antigen DQA from genomic DNA and determination of 25 new SLA class II haplotypes
- Authors
- Le, M. T.; Choi, H.; Choi, M. -K.; Nguyen, D. T.; Kim, J. -H.; Seo, H. G.; Cha, S. -Y.; Seo, K.; Chun, T.; Schook, L. B.; Park, C.
- Issue Date
- 12월-2012
- Publisher
- WILEY
- Keywords
- direct sequencing; haplotype; major histocompatibility complex; polymerase chain reaction; swine leukocyte antigen; SLA-DQA; swine
- Citation
- TISSUE ANTIGENS, v.80, no.6, pp.528 - 535
- Indexed
- SCIE
SCOPUS
- Journal Title
- TISSUE ANTIGENS
- Volume
- 80
- Number
- 6
- Start Page
- 528
- End Page
- 535
- ISSN
- 0001-2815
- Abstract
- We previously reported the development of genomic-DNA-based high-resolution genotyping methods for SLA-DQB1 and DRB1. Here, we report the successful typing of SLA-DQA using similar methodological principles. We designed a method for comprehensive genotyping of SLA-DQA using intronic sequence information of SLA-DQA exon 2 that we had obtained from 12 animals with different SLA-DQB1 genotypes. We expanded our typing to 76 selected animals with diverse DQB1 and DRB1 genotypes, 140 random animals from 7 pig breeds, and 3 wild boars. This resulted in the identification of 17 DQA alleles with 49 genotypes. Two new alleles were identified from wild boars. Combine with SLA-DQB1, and DRB1 typing results, we identified 34 SLA class II haplotypes including 25 that were previously unreported.
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Collections - Graduate School > Department of Biotechnology > 1. Journal Articles
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