Capsicum annuum WRKY transcription factor d (CaWRKYd) regulates hypersensitive response and defense response upon Tobacco mosaic virus infection
- Authors
- Huh, Sung Un; Choi, La Mee; Lee, Gil-Je; Kim, Young Jin; Paek, Kyung-Hee
- Issue Date
- 12월-2012
- Publisher
- ELSEVIER IRELAND LTD
- Keywords
- Capsicum annuum; WRKY; Hypersensitive response; Tobacco mosaic virus; Gene expression
- Citation
- PLANT SCIENCE, v.197, pp.50 - 58
- Indexed
- SCIE
SCOPUS
- Journal Title
- PLANT SCIENCE
- Volume
- 197
- Start Page
- 50
- End Page
- 58
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/106886
- DOI
- 10.1016/j.plantsci.2012.08.013
- ISSN
- 0168-9452
- Abstract
- WRKY transcription factors regulate biotic, abiotic, and developmental processes. In terms of plant defense, WRKY factors have important roles as positive and negative regulators via transcriptional regulation or protein-protein interaction. Here, we report the characterization of the gene encoding Capsicum annuum WRKY transcription factor d (CaWRKYd) isolated from microarray analysis in the Tobacco mosaic virus (TMV)-P-0-inoculated hot pepper plants. CaWRKYd belongs to the WRKY Ila group, a very small clade in the WRKY subfamily, and WRKY ha group has positive/negative regulatory roles in Arabidopsis and rice. CaWRKYd transcripts were induced by various plant defense-related hormone treatments and TMV-P-0 inoculation. Silencing of CaWRKYd affected TMV-P-0-mediated hypersensitive response (HR) cell death and accumulation of TMV-P-0 coat protein in local and systemic leaves. Furthermore, expression of some pathogenesis-related (PR) genes and HR-related genes was reduced in the CaWRKYd-silenced plants compared with TRV2 vector control plants upon TMV-P-0 inoculation. CaWRKYd was confirmed to bind to the W-box. Thus CaWRKYd is a newly identified Capsicum annuum WRKY transcription factor that appears to be involved in TMV-P-0-mediated HR cell death by regulating downstream gene expression. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
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