Ethanol-Dispersed Polymer Nanofibers as a Highly Selective Cell Isolation and Release Platform for CD4(+) T Lymphocytes
- Authors
- Jun, Seung-Hyun; Kim, Kwanghee; An, Hyo Jin; Kim, Byoung Chan; Sonn, Chung Hee; Kim, Miju; Doh, Junsang; Yee, Cassian; Lee, Kyung-Mi; Kim, Jungbae
- Issue Date
- 7-11월-2012
- Publisher
- WILEY-V C H VERLAG GMBH
- Keywords
- electrospun polymer nanofibers; ethanol dispersion; CD4+T lymphocyte; cell separation; cell transplantation
- Citation
- ADVANCED FUNCTIONAL MATERIALS, v.22, no.21, pp.4448 - 4455
- Indexed
- SCIE
SCOPUS
- Journal Title
- ADVANCED FUNCTIONAL MATERIALS
- Volume
- 22
- Number
- 21
- Start Page
- 4448
- End Page
- 4455
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/106952
- DOI
- 10.1002/adfm.201200657
- ISSN
- 1616-301X
- Abstract
- A novel cell isolation and release platform using electrospun polystyrene-poly(styrene-co-maleic anhydride) (PS-PSMA) nanofibers is presented. Ethanol treatment of PS-PSMA nanofibers, employed for the purpose of sterilization, significantly increases their inter-fiber space for both antibody conjugation and subsequent cell separation. For the selective isolation of CD4(+) T cells from heterogeneous mixtures of mouse lymph nodes, capture efficiencies of up to 100% are achieved while maintaining cellular integrity and viability. Once captured, CD4(+) T lymphocytes can also be released from the NF scaffolds, further demonstrating its potential functionality as an immune cell-supporting and releasing matrix. This is the first demonstration of lymphocyte-culture scaffolds enabling selective isolation, accommodation, and sustained release of CD4(+) T cells for the purpose of cell therapies.
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Collections - College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles
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