Do microRNA 96, 145 and 221 expressions really aid in the prognosis of prostate carcinoma?
- Authors
- Kang, Sung Gu; Ha, Young Ran; Kim, Seo Jin; Kang, Seok Ho; Park, Hong Seok; Lee, Jeong Gu; Cheon, Jun; Kim, Chul Hwan
- Issue Date
- 9월-2012
- Publisher
- ACTA PHARMACOLOGICA SINICA
- Keywords
- microRNA; prognosis; prostate cancer; recurrence
- Citation
- ASIAN JOURNAL OF ANDROLOGY, v.14, no.5, pp.752 - 757
- Indexed
- SCIE
SCOPUS
- Journal Title
- ASIAN JOURNAL OF ANDROLOGY
- Volume
- 14
- Number
- 5
- Start Page
- 752
- End Page
- 757
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/107598
- DOI
- 10.1038/aja.2012.68
- ISSN
- 1008-682X
- Abstract
- MicroRNAs (miRs) are small noncoding RNAs that have been reported to be promising diagnostic tools. We used quantitative real-time reverse transcription PCR (RT-qPCR) to analyze differentially expressed miRNAs in prostate tumor samples to determine its prognostic value. From 2007 to 2009, tumor tissues were obtained from 73 radical prostatectomy specimens. Differentially expressed miR-96, -145 and -221 were validated by TaqMan RT-qPCR using all 73 tissues. The prognostic value was assessed in terms of biochemical recurrence using Kaplan-Meier and Cox regression analyses. For our patient cohort, the mean age was 64.7 years (50-76 years) and the mean prostate-specific antigen (PSA) was 7.5 ng ml(-1). During the follow-up period (mean, 19.4 months), 14 of 73 (19.2%) patients developed biochemical recurrence. Expression of miR-96, -145 and -221 correlated strongly with each other, but there were no correlations between miRNA expression and clinicopathologic parameters. Kaplan-Meier survival curves using the log-rank test showed a decreased biochemical recurrence-free interval with pathologic stage (P<0.001). In addition, patients with Gleason scores over 8, compared with those with a Gleason score of 6, showed a decreased biochemical recurrence-free interval in Kaplan-Meier analysis (P=0.001). However, expression of miR-96, -145 and -221 did not correlate with the biochemical recurrence interval in Kaplan-Meier survival curves or by multivariate analysis using the Cox proportional hazard regression model, either. In conclusion, we did not observe a significant correlation between the expression of miR-96, -145 and -221 and clinicopathologic parameters. To utilize miRNA as a diagnostic tool in clinical practice, more research is needed to understand miRNA mechanisms, identify miRNA targets, and further characterize miRNA function. Asian Journal of Andrology (2012) 14, 752-757; doi:10.1038/aja.2012.68; published online 6 August 2012
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