beta-Galactosidase-immobilised microreactor fabricated using a novel technique for enzyme immobilisation and its application for continuous synthesis of lactulose
DC Field | Value | Language |
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dc.contributor.author | Song, Yoon Seok | - |
dc.contributor.author | Shin, Hyun Yong | - |
dc.contributor.author | Lee, Jin Young | - |
dc.contributor.author | Park, Chulhwan | - |
dc.contributor.author | Kim, Seung Wook | - |
dc.date.accessioned | 2021-09-06T16:55:59Z | - |
dc.date.available | 2021-09-06T16:55:59Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2012-08-01 | - |
dc.identifier.issn | 0308-8146 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/107732 | - |
dc.description.abstract | In this study, several surface functionalisation techniques were used to immobilise beta-galactosidase in a microreactor. beta-Galactosidase was pretreated with lactose before immobilisation, and functionalised multi-walled carbon nanotubes (MWNTs), DNA-wrapped single-walled carbon nanotubes and glutaraldehyde as a linker were used to immobilise beta-galactosidase on a microchannel surface. When functionalised MWNTs were used as a linker for immobilisation of pretreated beta-galactosidase, the enzyme microreactor showed the highest performance. The K-m(app) and V-max were 2.84 mM and 0.52 mM/min, respectively, and the conversion of o-nitrophenyl-beta-D-galactopyranoside (ONPG) reached 78.3% during the continuous flow reaction at a flow rate of 2.5 mu L/min. In an enzyme microreactor, continuous synthesis of lactulose was performed, and the lactulose concentration was maintained at about 1.29 g/L for 48 h. (C) 2012 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | MICROFLUIDIC DEVICES | - |
dc.subject | CARBON NANOTUBES | - |
dc.subject | REACTOR | - |
dc.subject | PERFORMANCE | - |
dc.subject | FUNCTIONALIZATION | - |
dc.title | beta-Galactosidase-immobilised microreactor fabricated using a novel technique for enzyme immobilisation and its application for continuous synthesis of lactulose | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Seung Wook | - |
dc.identifier.doi | 10.1016/j.foodchem.2012.01.096 | - |
dc.identifier.scopusid | 2-s2.0-84862795741 | - |
dc.identifier.wosid | 000302986400001 | - |
dc.identifier.bibliographicCitation | FOOD CHEMISTRY, v.133, no.3, pp.611 - 617 | - |
dc.relation.isPartOf | FOOD CHEMISTRY | - |
dc.citation.title | FOOD CHEMISTRY | - |
dc.citation.volume | 133 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 611 | - |
dc.citation.endPage | 617 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalResearchArea | Nutrition & Dietetics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Nutrition & Dietetics | - |
dc.subject.keywordPlus | MICROFLUIDIC DEVICES | - |
dc.subject.keywordPlus | CARBON NANOTUBES | - |
dc.subject.keywordPlus | REACTOR | - |
dc.subject.keywordPlus | PERFORMANCE | - |
dc.subject.keywordPlus | FUNCTIONALIZATION | - |
dc.subject.keywordAuthor | Enzyme microreactor | - |
dc.subject.keywordAuthor | beta-Galactosidase | - |
dc.subject.keywordAuthor | Functionalised multi-walled carbon nanotubes | - |
dc.subject.keywordAuthor | Pretreatment | - |
dc.subject.keywordAuthor | Lactulose | - |
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