beta-Galactosidase-immobilised microreactor fabricated using a novel technique for enzyme immobilisation and its application for continuous synthesis of lactulose
- Authors
- Song, Yoon Seok; Shin, Hyun Yong; Lee, Jin Young; Park, Chulhwan; Kim, Seung Wook
- Issue Date
- 1-8월-2012
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Enzyme microreactor; beta-Galactosidase; Functionalised multi-walled carbon nanotubes; Pretreatment; Lactulose
- Citation
- FOOD CHEMISTRY, v.133, no.3, pp.611 - 617
- Indexed
- SCIE
SCOPUS
- Journal Title
- FOOD CHEMISTRY
- Volume
- 133
- Number
- 3
- Start Page
- 611
- End Page
- 617
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/107732
- DOI
- 10.1016/j.foodchem.2012.01.096
- ISSN
- 0308-8146
- Abstract
- In this study, several surface functionalisation techniques were used to immobilise beta-galactosidase in a microreactor. beta-Galactosidase was pretreated with lactose before immobilisation, and functionalised multi-walled carbon nanotubes (MWNTs), DNA-wrapped single-walled carbon nanotubes and glutaraldehyde as a linker were used to immobilise beta-galactosidase on a microchannel surface. When functionalised MWNTs were used as a linker for immobilisation of pretreated beta-galactosidase, the enzyme microreactor showed the highest performance. The K-m(app) and V-max were 2.84 mM and 0.52 mM/min, respectively, and the conversion of o-nitrophenyl-beta-D-galactopyranoside (ONPG) reached 78.3% during the continuous flow reaction at a flow rate of 2.5 mu L/min. In an enzyme microreactor, continuous synthesis of lactulose was performed, and the lactulose concentration was maintained at about 1.29 g/L for 48 h. (C) 2012 Elsevier Ltd. All rights reserved.
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