Direct observation of the formation of DNA triplexes by single-molecule FRET measurements
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Il Buem | - |
dc.contributor.author | Lee, Ja Yil | - |
dc.contributor.author | Lee, Nam-Kyung | - |
dc.contributor.author | Hong, Seok-Cheol | - |
dc.date.accessioned | 2021-09-06T18:23:22Z | - |
dc.date.available | 2021-09-06T18:23:22Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2012-07 | - |
dc.identifier.issn | 1567-1739 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/108102 | - |
dc.description.abstract | In this report we investigated the effects of various biological and chemical factors (DNA sequence, pH, ions, and molecularity) on the formation of DNA triplexes through single-molecule FRET technique. Using this method, we determined how the third strand bound to a DNA duplex and how stable the triplex structure was under various conditions. From this study, we not only verified a variety of well-known features of DNA triplex but also discovered or experimentally supported several interesting behaviors: at neutral pH, a pyrimidine-motif triplex can be formed; the parallel arrangement was not only possible but also dominant over the antiparallel arrangement for a purine-motif triplex. We demonstrated that our method is a versatile analytical tool in studying structural aspects of nucleic acids, particularly non-classical DNA structures, and provides insights into physical mechanism of such structures. (C) 2012 Elsevier B. V. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | RECOGNITION | - |
dc.subject | COMPLEX | - |
dc.subject | PH | - |
dc.title | Direct observation of the formation of DNA triplexes by single-molecule FRET measurements | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hong, Seok-Cheol | - |
dc.identifier.doi | 10.1016/j.cap.2011.12.026 | - |
dc.identifier.scopusid | 2-s2.0-84862812216 | - |
dc.identifier.wosid | 000303129800005 | - |
dc.identifier.bibliographicCitation | CURRENT APPLIED PHYSICS, v.12, no.4, pp.1027 - 1032 | - |
dc.relation.isPartOf | CURRENT APPLIED PHYSICS | - |
dc.citation.title | CURRENT APPLIED PHYSICS | - |
dc.citation.volume | 12 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 1027 | - |
dc.citation.endPage | 1032 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001682590 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | PH | - |
dc.subject.keywordAuthor | DNA triplex | - |
dc.subject.keywordAuthor | H-DNA | - |
dc.subject.keywordAuthor | Single-molecule FRET | - |
dc.subject.keywordAuthor | pH | - |
dc.subject.keywordAuthor | Strand alignment | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.