Direct observation of the formation of DNA triplexes by single-molecule FRET measurements
- Authors
- Lee, Il Buem; Lee, Ja Yil; Lee, Nam-Kyung; Hong, Seok-Cheol
- Issue Date
- 7월-2012
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- DNA triplex; H-DNA; Single-molecule FRET; pH; Strand alignment
- Citation
- CURRENT APPLIED PHYSICS, v.12, no.4, pp.1027 - 1032
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- CURRENT APPLIED PHYSICS
- Volume
- 12
- Number
- 4
- Start Page
- 1027
- End Page
- 1032
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/108102
- DOI
- 10.1016/j.cap.2011.12.026
- ISSN
- 1567-1739
- Abstract
- In this report we investigated the effects of various biological and chemical factors (DNA sequence, pH, ions, and molecularity) on the formation of DNA triplexes through single-molecule FRET technique. Using this method, we determined how the third strand bound to a DNA duplex and how stable the triplex structure was under various conditions. From this study, we not only verified a variety of well-known features of DNA triplex but also discovered or experimentally supported several interesting behaviors: at neutral pH, a pyrimidine-motif triplex can be formed; the parallel arrangement was not only possible but also dominant over the antiparallel arrangement for a purine-motif triplex. We demonstrated that our method is a versatile analytical tool in studying structural aspects of nucleic acids, particularly non-classical DNA structures, and provides insights into physical mechanism of such structures. (C) 2012 Elsevier B. V. All rights reserved.
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