The Effect of Epidural Resiniferatoxin in the Neuropathic Pain Rat Model
- Authors
- Lee, Mi Geum; Huh, Billy K.; Choi, Sang Sik; Lee, Dong Kyu; Lim, Byung Gun; Lee, Mi Kyoung
- Issue Date
- 7월-2012
- Publisher
- AM SOC INTERVENTIONAL PAIN PHYSICIANS
- Keywords
- Epidural administration; mechanical allodynia; mechanical hypersensitivity; resiniferatoxin; sedation; spinal nerve ligation rat model; thermal hyperalgesia
- Citation
- PAIN PHYSICIAN, v.15, no.4, pp.287 - 296
- Indexed
- SCIE
SCOPUS
- Journal Title
- PAIN PHYSICIAN
- Volume
- 15
- Number
- 4
- Start Page
- 287
- End Page
- 296
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/108126
- ISSN
- 1533-3159
- Abstract
- Background: Resiniferatoxin (RTX) is a potent synthetic agonist for transient receptor potential vanilloid subtype 1 (TRPV1), which has a selectivity for antinociception. The analgesic effect of epidural RTX in a rat model of neuropathic pain has not yet been studied. Objectives: The purpose of this study was to evaluate the analgesic effect of epidural RTX on neuropathic pain in a rat model to mechanical and thermal stimulation. The dose-related behavior changes and side effects were also studied. Study design: A randomized, experimental trial. Setting: Department of Anesthesiology and Pain Medicine, Korea University Guro Hospital Methods: A spinal nerve ligation model was prepared using male Sprague-Dawley rats (7 weeks old, weight 230-250 g). An epidural catheter was placed at the L4-L5 level. Each study group (n = 6) received a different dose of RTX: 100 ng, 500 ng, 1 mu g, 2 mu g, 4 mu g and 10 mu g. All substances were administered in 20 mu L volume doses. The control group (n = 6) received 20 mu L of normal saline. We evaluated the response to mechanical and thermal stimuli as well as the sedation score at both short-term (3 hours) and long-term (20 days) after the epidural RTX injection. Results: Prolonged analgesia to thermal stimulation was preceded by a transient dose-dependent hyperalgesia (500 ng, 1 mu g) or sedation (>= 2 mu g) during the initial 60 minutes after RTX administration. Marked sedation and hyperventilation were noted at higher doses (>= 2 mu g), while 2 out of 6 rats died with a 10 mu g dose. ED50 for epidural RTX was 265 ng (95% confidence interval 216.1-324.9 ng). The increased latency to thermal stimulation continued for 20 days at RTX >= 1 mu g. But the threshold to mechanical stimulation increased only in the acute period and returned to the baseline after 3-5 days, regardless of the administered dose. Limitations: A histological examination by electron-microscopic staining was not performed. The observation period was not very long (20 days). Conclusion: RTX has potential to be used in an epidural route for neuropathic pain in a rat model with a relatively small amount, which produces transitory improvement of mechanical hypersensitivity and prolonged thermal analgesic response.
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