Immobilized cell microchannel bioreactor for evaluating fermentation characteristics of mixed substrate consumption and product formation
- Authors
- Seo, Tae Young; Eum, Ki Won; Han, Sung Ok; Kim, Seung Wook; Kim, Ji Hyeon; Song, Kwang Ho; Choe, Jaehoon
- Issue Date
- Jun-2012
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Continuous fermentation; Saccharomyces cerevisiae; Pichia stipitis; Immobilized cell microchannel bioreactor; Catabolite repression
- Citation
- PROCESS BIOCHEMISTRY, v.47, no.6, pp.1011 - 1015
- Indexed
- SCIE
SCOPUS
- Journal Title
- PROCESS BIOCHEMISTRY
- Volume
- 47
- Number
- 6
- Start Page
- 1011
- End Page
- 1015
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/108252
- DOI
- 10.1016/j.procbio.2012.03.019
- ISSN
- 1359-5113
- Abstract
- An immobilized cell microchannel bioreactor was designed to test continuous fermentation. The fermentation set-up included a bottom hydrophilic quartz channel to immobilize cells using 0.4 wt% polyethyleneimine and a top channel designed to continuously remove metabolically generated carbon dioxide using hydrophobic polypropylene. To evaluate fermentation characteristics of immobilized cells, ethanol fermentation was carried out using Saccharomyces cerevisiae and Pichia stipitis. The immobilized cell microchannel bioreactor was used to identify long-term activity of immobilized S. cerevisiae cells. The continuous flow microchannel bioreactor was operated stably over a period of 1 month. The immobilized cell microchannel bioreactor was used to examine the characteristics cells that consumed mixed substrates. The concentration ratio of glucose to xylose for simultaneous utilization of hemicellulosic sugars was evaluated using the microchannel bioreactor and the results were compared with those obtained by using conventional batch fermentation with P. stipitis. (C) 2012 Elsevier Ltd. All rights reserved.
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Collections - Graduate School > Department of Biotechnology > 1. Journal Articles
- College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles
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