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Characterization of a recombinant endo-type alginate lyase (Alg7D) from Saccharophagus degradans
- Authors
- Kim, Hee Taek; Ko, Hyeok-Jin; Kim, Nahyun; Kim, Duwoon; Lee, Dongho; Choi, In-Geol; Woo, Hee Chul; Kim, Myoung Dong; Kim, Kyoung Heon
- Issue Date
- Jun-2012
- Publisher
- SPRINGER
- Keywords
- Alg7d; Endo-type alginate lyase; Polysaccharide lyase-7; Saccharophagus degradans
- Citation
- BIOTECHNOLOGY LETTERS, v.34, no.6, pp.1087 - 1092
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOTECHNOLOGY LETTERS
- Volume
- 34
- Number
- 6
- Start Page
- 1087
- End Page
- 1092
- ISSN
- 0141-5492
- Abstract
- A gene, alg7D, from Saccharophagus degradans, coding for a putative alginate lyase belonging to the family of polysaccharide lyase-7, was overexpressed in Escherichia coli. The properties of the recombinant Alg7D were characterized. The enzyme endolytically depolymerized alginate by beta-elimination into oligo-alginates with degrees of polymerization of 2-5. Its activity was maximal at 50A degrees C and pH 7 and was slightly increased in the presence of Na+. The K (M) , V (max) , k (cat) , and k (cat) /K (M) values were: 3 mg ml(-1), 6.2 U mg(-1), 1.9 x 10(-2) s(-1), and 6.3 x 10(-3) mg(-1) ml s(-1), respectively.
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Related Researcher
- Kim, Kyoung Heon
- Department of Biotechnology