Density-dependent separation of encapsulated cells in a microfluidic channel by using a standing surface acoustic wave
DC Field | Value | Language |
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dc.contributor.author | Nam, Jeonghun | - |
dc.contributor.author | Lim, Hyunjung | - |
dc.contributor.author | Kim, Choong | - |
dc.contributor.author | Kang, Ji Yoon | - |
dc.contributor.author | Shin, Sehyun | - |
dc.date.accessioned | 2021-09-06T19:21:13Z | - |
dc.date.available | 2021-09-06T19:21:13Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2012-06 | - |
dc.identifier.issn | 1932-1058 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/108317 | - |
dc.description.abstract | This study presents a method for density-based separation of monodisperse encapsulated cells using a standing surface acoustic wave (SSAW) in a microchannel. Even though monodisperse polymer beads can be generated by the state-of-the-art technology in microfluidics, the quantity of encapsulated cells cannot be controlled precisely. In the present study, mono-disperse alginate beads in a laminar flow can be separated based on their density using acoustophoresis. A mixture of beads of equal sizes but dissimilar densities was hydrodynamically focused at the entrance and then actively driven toward the sidewalls by a SSAW. The lateral displacement of a bead is proportional to the density of the bead, i.e., the number of encapsulated cells in an alginate bead. Under optimized conditions, the recovery rate of a target bead group (large-cell-quantity alginate beads) reached up to 97% at a rate of 2300 beads per minute. A cell viability test also confirmed that the encapsulated cells were hardly damaged by the acoustic force. Moreover, cell-encapsulating beads that were cultured for 1 day were separated in a similar manner. In conclusion, this study demonstrated that a SSAW can successfully separate monodisperse particles by their density. With the present technique for separating cell-encapsulating beads, the current cell engineering technology can be significantly advanced. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4718719] | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER INST PHYSICS | - |
dc.subject | EMBRYONIC STEM-CELLS | - |
dc.subject | HYALURONIC-ACID | - |
dc.subject | EFFICIENT FORMATION | - |
dc.subject | SIZE | - |
dc.subject | HYDROGELS | - |
dc.subject | ALGINATE | - |
dc.subject | BODIES | - |
dc.subject | DROPLETS | - |
dc.subject | TRANSPLANTATION | - |
dc.subject | EMULSIFICATION | - |
dc.title | Density-dependent separation of encapsulated cells in a microfluidic channel by using a standing surface acoustic wave | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Shin, Sehyun | - |
dc.identifier.doi | 10.1063/1.4718719 | - |
dc.identifier.scopusid | 2-s2.0-84863193401 | - |
dc.identifier.wosid | 000305839800031 | - |
dc.identifier.bibliographicCitation | BIOMICROFLUIDICS, v.6, no.2 | - |
dc.relation.isPartOf | BIOMICROFLUIDICS | - |
dc.citation.title | BIOMICROFLUIDICS | - |
dc.citation.volume | 6 | - |
dc.citation.number | 2 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Physics, Fluids & Plasmas | - |
dc.subject.keywordPlus | EMBRYONIC STEM-CELLS | - |
dc.subject.keywordPlus | HYALURONIC-ACID | - |
dc.subject.keywordPlus | EFFICIENT FORMATION | - |
dc.subject.keywordPlus | SIZE | - |
dc.subject.keywordPlus | HYDROGELS | - |
dc.subject.keywordPlus | ALGINATE | - |
dc.subject.keywordPlus | BODIES | - |
dc.subject.keywordPlus | DROPLETS | - |
dc.subject.keywordPlus | TRANSPLANTATION | - |
dc.subject.keywordPlus | EMULSIFICATION | - |
dc.subject.keywordAuthor | biological techniques | - |
dc.subject.keywordAuthor | bioMEMS | - |
dc.subject.keywordAuthor | cellular biophysics | - |
dc.subject.keywordAuthor | flow separation | - |
dc.subject.keywordAuthor | laminar flow | - |
dc.subject.keywordAuthor | microchannel flow | - |
dc.subject.keywordAuthor | surface acoustic waves | - |
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